Document Detail

Effects of blood sampling technique and exercise on plasma androgens in female rats.
MedLine Citation:
PMID:  1569857     Owner:  NLM     Status:  MEDLINE    
Plasma concentrations of testosterone, androstenedione, and dihydrotestosterone rise during exercise in women. The purpose of this study was to evaluate exercise-induced changes in these three androgens in female rats to see if the rat may be used as a model for studying these responses. Because some androgen originates in the adrenal cortex, and the adrenocortical axis is stress-responsive, blood samples taken by cardiac puncture under halothane anesthesia, a potentially stressful technique, were compared with those taken by decapitation, which should not elicit a stress response. Blood was collected from sedentary rats at rest and from exercise-trained rats after 30 min of running. Half the samples were taken by each technique. Sampling technique did not affect androgen levels. Combining data from both techniques, testosterone was similar in rats at rest (503 pmol.l-1 +/- 62 (SE), N = 20) and post-exercise (500 +/- 31, N = 17). Androstenedione was higher post-exercise (1648 pmol.l-1 +/- 248, N = 17) than at rest (866 +/- 115, N = 18; P less than 0.009). Dihydrotestosterone was also higher post-exercise (452 pmol.l-1 +/- 31, N = 17) than at rest (324 +/- 41, N = 20; P less than 0.05). It is concluded that: 1) cardiac puncture with halothane anesthesia is acceptable for studying androgens in female rats, and 2) the female rat may be useful for studying the androstenedione and dihydrotestosterone responses to exercise, but is not appropriate for studying testosterone exercise responses.
K A Carlberg; B L Alvin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  24     ISSN:  0195-9131     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  1992 May 
Date Detail:
Created Date:  1992-05-28     Completed Date:  1992-05-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  610-4     Citation Subset:  IM; S    
Department of Biology, Eastern Washington University, Cheney 99004.
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MeSH Terms
Androgens / blood*
Androstenedione / blood
Blood Specimen Collection / methods*
Dihydrotestosterone / blood
Models, Biological
Physical Conditioning, Animal*
Rats, Inbred Strains
Testosterone / blood
Reg. No./Substance:
0/Androgens; 521-18-6/Dihydrotestosterone; 58-22-0/Testosterone; 63-05-8/Androstenedione

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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