| Effects of biotin deficiency on embryonic development in mice. | |
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MedLine Citation:
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PMID: 18752930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The purpose of this investigation was to determine the effects of biotin deficiency on maternal metabolism and embryonic development in pregnant mouse dams. METHODS: The pregnant mice were randomly assigned to one of three dietary groups and given a biotin-deficient diet, biotin-supplemented diet, or biotin-control diet during gestation. On days of gestation (dgs) 0, 4, 8, 12, and 16, organic acids including 3-hydroxyisovaleric acid in urine were discovered by high-performance liquid chromatography, and the biotin level in the serum and urine was determined by a bioassay. On dg 18, fetuses were examined for morphologic development. RESULTS: In the biotin-deficient group, biotin excretion in urine decreased on dg 4 and was subsequently below the lower limit, whereas the urinary concentration of 3-hydroxyisovaleric acid increased after dg 12. In contrast, the biotin concentration in urine significantly increased on dgs 4, 8 and 12 in the biotin-supplemented group, but decreased on dg 16 in the biotin-supplemented and biotin-control groups. The urinary excretion of pyruvic acid in the biotin-deficient group was significantly higher than that in the biotin-supplemented group throughout the entire gestation. These concentrations in urine significantly increased on dg 16 compared with dg 0. The inhibition of embryonic development and external malformations such as cleft palate (100%), micrognathia (100%), and micromelia (91.4%) were also detected in biotin-deficient fetuses. CONCLUSION: These findings indicated that, as the requirement of biotin increases during gestation and/or embryonic development, a large amount of biotin is necessary for maintaining normal reproductive performance during the late stage of gestation. |
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Authors:
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Toshiaki Watanabe; Yoshiko Nagai; Ayumi Taniguchi; Shuhei Ebara; Sachiko Kimura; Toru Fukui |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-08-26 |
Journal Detail:
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Title: Nutrition (Burbank, Los Angeles County, Calif.) Volume: 25 ISSN: 0899-9007 ISO Abbreviation: Nutrition Publication Date: 2009 Jan |
Date Detail:
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Created Date: 2008-12-08 Completed Date: 2009-03-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8802712 Medline TA: Nutrition Country: United States |
Other Details:
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Languages: eng Pagination: 78-84 Citation Subset: IM |
Affiliation:
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Department of Dietary Environment Analysis, School of Human Science and Environment, Himeji Institute of Technology, University of Hyogo, Himeji, Japan. watanabe@shse.u-hyogo.ac.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biotin / blood, deficiency*, metabolism*, urine Chromatography, High Pressure Liquid / methods Congenital Abnormalities / epidemiology, etiology* Embryonic Development* / drug effects Energy Metabolism / physiology* Female Fetus / metabolism Gestational Age Male Mice Mice, Inbred ICR Nutritional Requirements Pregnancy Pregnancy Outcome Pregnancy, Animal / metabolism* Random Allocation Valerates / urine |
| Chemical | |
Reg. No./Substance:
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0/Valerates; 58-85-5/Biotin; 625-08-1/beta-hydroxyisovaleric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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