Document Detail


Effects of biofilm formation on haemodialysis monitor disinfection.
MedLine Citation:
PMID:  13679487     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Biofilms are composed of communities of micro-organisms adhering to essentially any surface. We evaluated whether biofilm formation in the hydraulic circuit of a purposely contaminated haemodialysis monitor would modify the efficacy of different disinfection modalities against bacteria and endotoxin concentrations. METHODS: A water-borne Pseudomonas aeruginosa (109) suspension was recirculated for 1 h and was left standing for 72 h (stationary phase) in the hydraulic circuit of the monitor. The monitor was then washed and disinfected by different physical (heat, 85 degrees C) or chemical (hypochlorite or peracetic acid) disinfection modalities (protocol A). In protocol B, the bacterial suspension was also recirculated for 1 h, but the monitor was then immediately washed and disinfected by different chemical disinfection modalities (hypochlorite or peracetic acid). RESULTS: Biofilm formation was revealed by scanning and confocal laser electron microscopy after the stationary phase (protocol A), but was absent when the monitor was immediately washed and disinfected (protocol B). In the presence of biofilm (protocol A), heat in association with citric acid was the most effective modality for reducing both colony forming units and endotoxin concentrations, whereas heat by itself was the least effective method of disinfection. Dwelling (60 h) with diluted peracetic acid completely prevented the formation of biofilm. In the absence of biofilm (protocol B), chemical disinfection proved to be effective against both colony forming units and endotoxin concentrations. CONCLUSIONS: We found that biofilm formation may markedly reduce the efficacy of presently available disinfection modalities. Therefore, different disinfection modalities and the combined action of descaling (by citric acid) and disinfection (physical/chemical agents) should be used periodically in haemodialysis monitors. In addition, dwelling with diluted peracetic acid should be adopted whenever monitors are not in use.
Authors:
Gianni Cappelli; Luisa Sereni; Maria Grazia Scialoja; Massimo Morselli; Salvatore Perrone; Angela Ciuffreda; Massimo Bellesia; Paola Inguaggiato; Alberto Albertazzi; Ciro Tetta
Related Documents :
11140827 - Inhibiting effect of ammonia on citric acid-induced cough in pigs: a possible involveme...
22410487 - An integrated microfluidic system for rapid screening of alpha-fetoprotein-specific apt...
11058157 - Self-organizing biochemical cycles.
9890317 - Effects of hemodialyzer reuse on clearances of urea and beta2-microglobulin. the hemodi...
18786547 - Specific sequence combinations at parallel and antiparallel helix-helix interfaces.
23743267 - Isolation and structural characterization of a (kdo-isosteric) d-glycero-α-d-talo-oct-...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association     Volume:  18     ISSN:  0931-0509     ISO Abbreviation:  Nephrol. Dial. Transplant.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-09-18     Completed Date:  2004-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8706402     Medline TA:  Nephrol Dial Transplant     Country:  England    
Other Details:
Languages:  eng     Pagination:  2105-11     Citation Subset:  IM    
Affiliation:
Department of Nephrology, University Hospital of Modena, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Bacterial Infections / etiology,  prevention & control*
Biofilms*
Colony Count, Microbial
Disinfection / methods*
Equipment Contamination
Humans
Microscopy, Electron
Probability
Pseudomonas aeruginosa*
Renal Dialysis / adverse effects,  instrumentation*
Risk Assessment
Sensitivity and Specificity

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Optimal anticoagulation strategy in haemodialysis with heparin-coated polyacrylonitrile membrane.
Next Document:  Risk factors for increased variability in dialysis delivery in haemodialysis patients.