Document Detail


Effects of bioactive glass particles and their ionic products on intracellular concentrations.
MedLine Citation:
PMID:  12761833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Numerous studies have described the bioactive properties of glass particles in the SiO(2)-CaO-Na(2)O-P(2)O(5) system. This kind of material is capable of developing a direct contact with bone through dissolution and physicochemical reactions. We have investigated the influence of bioactive particles, and ionic products from the same particles, on the intracellular concentrations in monocyte cells, which are among the first cells to colonize implantation sites. The only way to access these concentrations and particularly diffusible ionic concentrations (potassium, sodium, and chlorine) is to use cryomethods coupled to electron probe microanalysis. We have paid particular attention to the potassium:sodium ratio, the most sensitive criterion of viability. We have cultured cells with bioactive glass particles and in a conditioned medium obtained from the dissolution of the glass particles in the standard medium. Our study demonstrates that cells cultured in a conditioned medium are more active than cells cultured in a standard medium, or cells exposed to bioactive particles, and particles are more toxic for cells than are ionic products.
Authors:
Patrice Laquerriere; Edouard Jallot; Laurence Kilian; Hicham Benhayoune; Gérard Balossier
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  65     ISSN:  1549-3296     ISO Abbreviation:  -     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-05-22     Completed Date:  2003-08-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  441-6     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 441-446, 2003
Affiliation:
INSERM-ERM 0203, Laboratoire de Microscopie Electronique, UFR Sciences, 21 rue Clément Ader, BP 138, 51685 Reims, Cedex 2, France.
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MeSH Terms
Descriptor/Qualifier:
Biocompatible Materials / chemistry*,  pharmacokinetics*
Bone Substitutes / chemistry,  pharmacokinetics
Cell Nucleus / metabolism
Cells, Cultured
Culture Media, Conditioned
Cytoplasm / metabolism
Glass / chemistry*
Humans
Materials Testing
Microscopy, Electron, Scanning
Monocytes / metabolism
Particle Size
Potassium / metabolism
Sodium / metabolism
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Bone Substitutes; 0/Culture Media, Conditioned; 7440-09-7/Potassium; 7440-23-5/Sodium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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