Document Detail


Effects of bile acids on iodide uptake and deoxyribonucleic acid synthesis in porcine thyroid cells in primary culture.
MedLine Citation:
PMID:  8936674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of bile acids on iodide uptake and DNA synthesis were studied in cultured porcine thyroid cells. All five bile acids, which are commonly found in serum, chenodeoxycholic acid (CDCA), cholic acid (CA), deoxycholic acid (DCA), lithocholic acid (LCA), and ursodeoxycholic acid (UDCA) dose-dependently inhibited both basal and TSH-induced iodide uptake at concentrations of 25-250 microM. Since CDCA is one of the two major primary endogenous bile acids, were studied mainly the effects of CDCA. The inhibitory effect of CDCA was detected after 24 h treatment of thyroid cells, and was dependent on the time of exposure up to 72 h. Treatment of thyroid cells with CDCA for 72 h inhibited cAMP production stimulated by 50 mU/L TSH or 0.5 mg/L forskolin and also inhibited iodide uptake induced by 0.5 mM 8-bromo cAMP or 0.5 mg/L forskolin. These results suggest that CDCA inhibits iodide uptake by decreasing cAMP production as well as post-cAMP generation. Bile acids except LCA stimulated [3H]thymidine incorporation into the thyroid cells by itself, indicating that the inhibitory effect of bile acids on iodide uptake is not due to cytotoxic effect. In conclusion, these findings suggest that the direct inhibition of thyroid function by bile acids might cause hypothyroidism in chronic liver disease.
Authors:
R Kanri; Y Takiyama; I Makino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Thyroid : official journal of the American Thyroid Association     Volume:  6     ISSN:  1050-7256     ISO Abbreviation:  Thyroid     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1997-03-28     Completed Date:  1997-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104317     Medline TA:  Thyroid     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  467-74     Citation Subset:  IM    
Affiliation:
Second Department of Internal Medicine, Asahikawa Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
8-Bromo Cyclic Adenosine Monophosphate / pharmacology
Animals
Bile Acids and Salts / pharmacology*
Cells, Cultured
Chenodeoxycholic Acid / pharmacology
Cholic Acid
Cholic Acids / pharmacology
Cyclic AMP / biosynthesis
DNA / biosynthesis*
Deoxycholic Acid / pharmacology
Forskolin / pharmacology
Iodides / metabolism*
Ion Transport / drug effects
Lithocholic Acid / pharmacology
Swine
Thyroid Gland / drug effects*,  metabolism*
Thyrotropin / pharmacology
Ursodeoxycholic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Cholic Acids; 0/Iodides; 128-13-2/Ursodeoxycholic Acid; 23583-48-4/8-Bromo Cyclic Adenosine Monophosphate; 434-13-9/Lithocholic Acid; 474-25-9/Chenodeoxycholic Acid; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; 81-25-4/Cholic Acid; 83-44-3/Deoxycholic Acid; 9002-71-5/Thyrotropin; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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