Document Detail


Effects of bee venom on the pro-inflammatory responses in RAW264.7 macrophage cell line.
MedLine Citation:
PMID:  15848037     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of this study is to elucidate the molecular mechanism of anti-inflammatory effect of bee venom (BV), which has been used for the treatment of various inflammatory diseases in oriental medicine. With this aim, we examined the effects of BV on the nitric oxide (NO) production by lipopolysaccharide (LPS) or sodium nitroprusside in RAW264.7 macrophages. We further investigated the effects of BV on the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor-kappaB (NF-kappaB) and mitogen-activated protein kinase (MAPK) with RT-PCR in LPS-stimulated RAW264.7 cells. BV suppressed the NO production and decreased the levels of iNOS, COX-2, NF-kappaB and MAPK mRNA in a dose-dependent manner. These results suggest that BV has an anti-inflammatory effect by inhibiting iNOS and COX-2 expression, possibly through suppression of NF-kappaB and MAPK expression.
Authors:
Hyoung-Seok Jang; Sun Kwang Kim; Jae-Bok Han; Hyun-Jong Ahn; Hyunsu Bae; Byung-Il Min
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  99     ISSN:  0378-8741     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-04-25     Completed Date:  2005-08-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  157-60     Citation Subset:  IM    
Affiliation:
Department of East-West Medicine, Graduate School, Kyung-Hee University, Seoul 130-701, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bee Venoms / pharmacology*
Cell Line
Cyclooxygenase 2
Dose-Response Relationship, Drug
Inflammation / pathology*
Lipopolysaccharides / pharmacology
Macrophages / drug effects*,  pathology*
Mice
Mitogen-Activated Protein Kinases / biosynthesis
NF-kappa B / biosynthesis
Nitric Oxide / biosynthesis
Nitric Oxide Synthase / biosynthesis
Nitric Oxide Synthase Type II
Nitroprusside / pharmacology
Prostaglandin-Endoperoxide Synthases / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Chemical
Reg. No./Substance:
0/Bee Venoms; 0/Lipopolysaccharides; 0/NF-kappa B; 10102-43-9/Nitric Oxide; 15078-28-1/Nitroprusside; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases; EC 2.7.11.24/Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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