Document Detail


Effects of basic fibroblast growth factor on bone formation in vitro.
MedLine Citation:
PMID:  3366907     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Basic fibroblast growth factor (bFGF) was studied for its effects on bone formation in cultured rat calvariae. bFGF at 0.1-100 ng/ml stimulated [3H]thymidine incorporation into DNA by up to 4.4-fold. bFGF also increased the number of colcemid-induced metaphase arrested cells and the DNA content. Transient (24 h) treatment with bFGF enhanced [3H]-proline incorporation into collagen 24-48 h after the factor was removed; this effect was DNA synthesis dependent and blocked by hydroxyurea. The collagen stimulated by bFGF was type I, and this effect was observed primarily in the periosteum-free bone. In contrast, continuous treatment with bFGF for 24-96 h inhibited [3H]proline incorporation into type I collagen. bFGF did not alter collagen degradation. In conclusion, bFGF stimulates calvarial DNA synthesis, which causes an increased number of collagen-synthesizing cells, but bFGF has a direct inhibitory effect on collagen synthesis.
Authors:
E Canalis; M Centrella; T McCarthy
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  81     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1988 May 
Date Detail:
Created Date:  1988-06-14     Completed Date:  1988-06-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1572-7     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine (Endocrine Section), Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bone Development / drug effects*
Collagen / biosynthesis,  metabolism
Culture Techniques
DNA / biosynthesis
Fibroblast Growth Factors / pharmacology*
Metaphase
Osteoblasts / drug effects,  metabolism*
Protein Biosynthesis
Rats
Recombinant Proteins / pharmacology
Grant Support
ID/Acronym/Agency:
AR21707/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Recombinant Proteins; 62031-54-3/Fibroblast Growth Factors; 9007-34-5/Collagen; 9007-49-2/DNA
Comments/Corrections

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