Document Detail


Effects of the augmenter of liver regeneration on the biological behavior of hepatocellular carcinoma.
MedLine Citation:
PMID:  19668879     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To take advantage of the small interfering ribonucleic acid (siRNA) targeting the human augmenter of liver regeneration (hALR) and anti-hALR monoclonal antibody (McAb) to inhibit the function of hALR, and to demonstrate whether the growth of hepatoma is influenced by siRNA targeting hALR and anti-hALR McAb through inhibiting expression of hALR. METHODS: This study was conducted in the Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing Medical University, China, between January 2005 and May 2007. We transfected siRNA plasmid pSIALR-A, which targeted the complementary deoxyribonucleic acid (cDNA) of hALR and the unrelated control plasmid pSIALR-B into human hepatocellular liver carcinoma cell line (HepG2) cells. Then, the proliferation of HepG2 cells, after being treated with pSIALR-A and anti-hALR McAb was detected. The growth of the xenograft tumor was observed after being treated with pSIALR-A and anti-hALR McAb in nude mice. RESULTS: We successfully constructed expressing plasmid pSIALR-A and pSIALR-B. The pSIALR-A inhibited the expression of hALR in HepG2 cells significantly. The siRNA targeting hALR and anti-hALR McAb inhibited obviously the growth of HepG2 cells in vitro. siRNA targeting hALR and anti-hALR McAb significantly inhibited the growth of xenograft tumor in 5 nude mice. Anti-hALR McAb inhibited apparently the autonomous growth of HepG2 cells. CONCLUSION: Our results demonstrated that anti-hALR McAb inhibited the autonomous growth of hepatoma cells obviously, moreover, hALR maintained the autonomous growth of hepatoma cells in vitro through an autocrine mechanism.
Authors:
Lin Tang; Hang Sun; Lin Zhang; Jian C Deng; Hui Guo; Ling Zhang; Qi Liu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Saudi medical journal     Volume:  30     ISSN:  0379-5284     ISO Abbreviation:  Saudi Med J     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-08-11     Completed Date:  2009-12-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909441     Medline TA:  Saudi Med J     Country:  Saudi Arabia    
Other Details:
Languages:  eng     Pagination:  1001-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Molecular Biology for Infectious Diseases, Ministry of Education, Institute for Viral Hepatitis, Chongqing University of Medical Sciences, Chongqing, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology
Carcinoma, Hepatocellular / pathology*
Cell Line, Tumor
Cell Proliferation / drug effects
Female
Immunohistochemistry
Liver Neoplasms / pathology*
Liver Regeneration / physiology*
Mice
Mice, Inbred BALB C
Mice, Nude
Proteins / physiology*
RNA, Small Interfering / genetics
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Proteins; 0/RNA, Small Interfering; 0/augmenter of liver regeneration factor

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