Document Detail

Effects of astragaloside IV on eosinophil activation induced by house dust mite allergen.
MedLine Citation:
PMID:  22505212     Owner:  NLM     Status:  MEDLINE    
Astragaloside IV (AS-IV) has been noted for its reduction of eosinophilic airway inflammation in a murine model of chronic asthma. To gain a better understanding of the mechanisms involved in this anti-inflammatory phenomenon, the effect of AS-IV on human blood eosinophils was studied in vitro. Eosinophils were isolated from the blood of patients with mild atopic asthma, preincubated with AS-IV for 1 h and stimulated in the presence or absence of the house dust mite allergen Dermatophagoides pteronyssinus (Der p) 1 for 4 h. The survival of the eosinophils at 48 h was investigated using trypan blue and the surface expression of CC chemokine receptor 3 (CCR3) and intercellular adhesion molecule-1 (ICAM-1) by the eosinophils was analyzed using flow cytometry. The secretion of cytokines in the supernatants and the chemotaxis of the eosinophils were measured by ELISA and the transwell system, respectively. Der p 1 was found to prolong the survival of the eosinophils. Similarly, the expression of CCR3 and ICAM-1, secretion of interleukin (IL)-1β, IL-5, tumor necrosis factor (TNF)-α and the granulocyte macrophage colony stimulating factor (GM-CSF) and transmigration of the eosinophils were increased in the presence of Der p 1. However, these inductive effects on the eosinophils were significantly inhibited by AS-IV (50 µg/ml). These findings suggest that AS-IV modulates eosinophil activation and trafficking in response to Der p 1 and may therefore be a useful therapeutic option in eosinophilic asthma.
Xiaoyan Gu; Desheng Jiang; Yuwei Wang; Qiang Du; Jiankang Cai
Publication Detail:
Type:  Journal Article     Date:  2012-04-12
Journal Detail:
Title:  Molecular medicine reports     Volume:  6     ISSN:  1791-3004     ISO Abbreviation:  Mol Med Rep     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-05-09     Completed Date:  2012-09-10     Revised Date:  2013-02-22    
Medline Journal Info:
Nlm Unique ID:  101475259     Medline TA:  Mol Med Rep     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  115-20     Citation Subset:  IM    
Department of Respiratory Medicine, The 454th Hospital of Chinese PLA, Nanjing, Jiangsu 210002, PR China.
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MeSH Terms
Allergens / immunology*
Anti-Inflammatory Agents / chemistry,  pharmacology*
Antigens, Dermatophagoides / immunology*
Arthropod Proteins / immunology*
Cell Survival / drug effects,  immunology
Cells, Cultured
Cysteine Endopeptidases / immunology*
Cytokines / biosynthesis
Drugs, Chinese Herbal / chemistry,  pharmacology*
Eosinophils / drug effects*,  immunology*,  metabolism
Intercellular Adhesion Molecule-1 / metabolism
Receptors, CCR3 / metabolism
Saponins / chemistry,  pharmacology*
Time Factors
Transendothelial and Transepithelial Migration / drug effects,  immunology
Triterpenes / chemistry,  pharmacology*
Reg. No./Substance:
0/Allergens; 0/Anti-Inflammatory Agents; 0/Antigens, Dermatophagoides; 0/Arthropod Proteins; 0/Cytokines; 0/Drugs, Chinese Herbal; 0/Receptors, CCR3; 0/Saponins; 0/Triterpenes; 126547-89-5/Intercellular Adhesion Molecule-1; 83207-58-3/astragaloside A; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.-/Dermatophagoides pteronyssinus antigen p 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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