Document Detail

Effects of aspirin and other nonsteroidal anti-inflammatory drugs on biofilms and planktonic cells of Candida albicans.
MedLine Citation:
PMID:  14693516     Owner:  NLM     Status:  MEDLINE    
Prostaglandins are now known to be produced by Candida albicans and may play an important role in fungal colonization. Their synthesis in mammalian cells is decreased by inhibitors of the cyclooxygenase isoenzymes required for prostaglandin formation. In the present study, a catheter disk model system was used to investigate the effects of nonsteroidal anti-inflammatory drugs (all cyclooxygenase inhibitors) on biofilm formation by three strains of C. albicans. Seven of nine drugs tested at a concentration of 1 mM inhibited biofilm formation. Aspirin, etodolac, and diclofenac produced the greatest effects, with aspirin causing up to 95% inhibition. Celecoxib, nimesulide, ibuprofen, and meloxicam also inhibited biofilm formation, but to a lesser extent. Aspirin was active against growing and fully mature (48-h) biofilms; its effect was dose related, and it produced significant inhibition (20 to 80%) at pharmacological concentrations. Simultaneous addition of prostaglandin E(2) abolished the inhibitory effect of 25 or 50 micro M aspirin. At 1 mM, aspirin reduced the viability of biofilm organisms to 1.9% of that of controls. Surviving cells had a wrinkled appearance, as judged by scanning electron microscopy, and consisted of both yeasts and hyphae. Treatment with other cyclooxygenase inhibitors, such as etodolac, resulted in biofilms that consisted almost entirely of yeast cells. In conventional assays for germ tube formation, these drugs produced significant inhibition, whereas aspirin had little effect. Our findings suggest that cyclooxygenase-dependent synthesis of fungal prostaglandin(s) is important for both biofilm development and morphogenesis in C. albicans and may act as a regulator in these physiological processes. Our results also demonstrate that aspirin possesses potent antibiofilm activity in vitro and could be useful in combined therapy with conventional antifungal agents in the management of some biofilm-associated Candida infections.
Mohammed A S Alem; L Julia Douglas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Antimicrobial agents and chemotherapy     Volume:  48     ISSN:  0066-4804     ISO Abbreviation:  Antimicrob. Agents Chemother.     Publication Date:  2004 Jan 
Date Detail:
Created Date:  2003-12-24     Completed Date:  2004-02-20     Revised Date:  2013-04-18    
Medline Journal Info:
Nlm Unique ID:  0315061     Medline TA:  Antimicrob Agents Chemother     Country:  United States    
Other Details:
Languages:  eng     Pagination:  41-7     Citation Subset:  IM    
Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, United Kingdom.
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MeSH Terms
Anti-Inflammatory Agents, Non-Steroidal / pharmacology*
Aspirin / pharmacology*
Biofilms / drug effects*,  growth & development
Candida albicans / drug effects*,  enzymology,  ultrastructure
Culture Media
Cyclooxygenase Inhibitors / pharmacology*
Dinoprostone / pharmacology
Dose-Response Relationship, Drug
Microbial Sensitivity Tests
Microscopy, Electron, Scanning
Prostaglandin-Endoperoxide Synthases / metabolism*
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Culture Media; 0/Cyclooxygenase Inhibitors; 363-24-6/Dinoprostone; 50-78-2/Aspirin; EC Synthases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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