Document Detail


Effects of ascorbic acid on retinal pigment epithelial cells.
MedLine Citation:
PMID:  11803483     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: Evaluation of effects of ascorbic acid on cell characteristics of dedifferentiated porcine retinal pigment epithelial (pRPE) cells. METHODS: pRPE cells were incubated in vitro with increasing concentrations of ascorbic acid (0.25-1.5 mMol). Cell proliferation was assayed by measuring the incorporation of 5-bromo-2'-deoxy-uridine (BrdU) into cellular DNA. Migration and contraction properties were studied on a cell permissive porous membrane and collagen gels, respectively. Phenotypic changes in response to ascorbic acid and its derivative ascorbic acid 2-phophate were evaluated by microscopy and indirect immunofluorescence. RESULTS: Ascorbic acid significantly inhibits cell proliferation, migration, and contraction in concentrations of 1 mMol or more. Under the influence of at least 1 mMol ascorbic acid dedifferentiated pRPE cells exhibited a pigmented status within 24 hours. Addition of 500 U/ml catalase prevented the antiproliferative effect of ascorbic acid and the formation of pigment. Concentrations of 0.5 mMol ascorbic acid as well as 1 mMol ascorbic acid 2-phosphate promoted differentiation of cell phenotype. Furthermore, ascorbic acid 2-phosphate supported the formation of in vivo-like epithelial structures. CONCLUSIONS: Ascorbic acid has an influence on vital cell characteristics such as proliferation, migration, contraction and differentiation of pRPE cells. As dedifferentiation of these cells is an integral part in the development of proliferative vitreoretinopathy (PVR), ascorbic acid should be taken into consideration as a supplement in the clinical management of this disease.
Authors:
J A Böhmer; B Sellhaus; N F Schrage
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Current eye research     Volume:  23     ISSN:  0271-3683     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  2001 Sep 
Date Detail:
Created Date:  2002-01-22     Completed Date:  2002-03-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  206-14     Citation Subset:  IM    
Affiliation:
Interdisciplinary Centre of Clinical Research (IZKF) BIOMAT, Technical University of Aachen, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Ascorbic Acid / pharmacology*
Bromodeoxyuridine / diagnostic use
Catalase / pharmacology
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Movement / drug effects
Cell Separation
Cells, Cultured
Collagen / metabolism
DNA / biosynthesis
Fluorescent Antibody Technique, Indirect
Pigment Epithelium of Eye / cytology,  drug effects*,  metabolism
Platelet-Derived Growth Factor / pharmacology
Swine
Chemical
Reg. No./Substance:
0/Platelet-Derived Growth Factor; 50-81-7/Ascorbic Acid; 59-14-3/Bromodeoxyuridine; 9007-34-5/Collagen; 9007-49-2/DNA; EC 1.11.1.6/Catalase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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