Document Detail

Effects of ascorbic acid on lead induced alterations of synaptic transmission and contractile features in murine dorsiflexor muscle.
MedLine Citation:
PMID:  12818354     Owner:  NLM     Status:  MEDLINE    
Lead is a common environmental toxin that affects neuromuscular junction and potentially might cause muscle weakness. Antioxidants like ascorbic acid may protect against lead induced myopathy. The present study measured isometric twitch tensions (evoked either directly by muscle stimulation or indirectly by nerve stimulation) to study effects of ascorbic acid on lead induced alterations at murine dorsiflexor skeletal muscle. Resting membrane potentials (RMPs), endplate potentials (EPPs) and miniature endplate potentials (MEPPs) were also recorded. Forty animals were divided into four groups of n = 10 each. (10 control, 10 lead alone, 10 ascorbic acid alone, 10 lead treated plus ascorbic acid). Lead (1 mg/kg) i.p, was administered daily for 2 weeks before the recording day and ascorbic acid (200 mg/kg, i.p) was given daily for 3 weeks prior to the experiment day. Lead treatment reduced twitch tension significantly (from 4.3 +/- 0.5 g to 2.7 +/- 0.2 g) and delayed half time of decay compared to the control. Similarly MEPPs frequencies were reduced following lead treatment. Application of ascorbic acid prevented twitch tension reduction in lead treated mice (3.3 +/- 0.3 g) and reversed lead induced delay in half time of decay. The negative actions of lead treatment on MEPPs frequencies were also modified with ascorbic acid. It appears that ascorbic acid exerts a protective role against lead induced peripheral nerve and muscle dysfunction. This effect of ascorbic acid on lead induced neuromyopathy is probably mediated via a free radical scavenging mechanism or modification of Ca(2+) homeostasis.
M Y Hasan; W B Alshuaib; S Singh; M A Fahim
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Life sciences     Volume:  73     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-06-23     Completed Date:  2003-07-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  1017-25     Citation Subset:  IM    
Department of Pharmacology, Faculty of Medicine, UAE University, PO Box 17666, Al Ain, United Arab Emirates.
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MeSH Terms
Antioxidants / pharmacology*
Ascorbic Acid / pharmacology*
Body Weight / drug effects
Isometric Contraction / drug effects
Lead / antagonists & inhibitors*,  blood,  toxicity*
Membrane Potentials / drug effects
Mice, Inbred C57BL
Motor Endplate / drug effects,  metabolism
Muscle Contraction / drug effects
Muscle, Skeletal / drug effects*,  innervation,  metabolism
Neurotransmitter Agents / metabolism
Synaptic Transmission / drug effects*
Reg. No./Substance:
0/Antioxidants; 0/Neurotransmitter Agents; 50-81-7/Ascorbic Acid; 7439-92-1/Lead

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