| Effects of arsenic exposure during the pre- and postnatal development on the puberty of female offspring. | |
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MedLine Citation:
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PMID: 20580540 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To evaluate if sub-chronic As exposure during the pre- and postnatal development causes disturbances in the puberty. Moreover, determine adverse effects of As on the ovarian follicle and adrenocortical cell maturation. METHODS: Females adult Wistar rats were exposed to sodium arsenite at 3ppm calculated as As in drinking water from mating, gestation. Following the birth, the female offspring continued exposured to As via lactation. Weaned pups received the same As treatment as mothers, until they were 1-4 months (mo) old. At these ages, blood sampling and tissue harvest were done. The tissues were fixed in situ with 4% paraformaldehyde in phosphate buffer. After the perfusion the ovaries, uterus, adrenal glands were harvested, dissected out, weighted. The ovaries and the adrenal glands were processed to paraffin and sectioned at 5μM and stained with hematoxylin and eosin for light microscopy. STATISTICAL ANALYSIS: Comparisons between groups were made by unpaired t-test or nonparametric Mann-Whitney test as appropriate. RESULTS: 100% As treated rats at 1 mo of age were at diestrous stage, with low estradiol E2. As treatment caused disturbances in the morphology of the ovarian cell consisting in DNA damage evidenced by picknotic chromatin, cariorexis, significant cytoplasmic vacuolization and also vasculature damaged. Arrest in follicle maturation was also present. CONCLUSIONS: We found that the onset of puberty in the As treated rats was 1 mo delayed since vagina was still closed, the vaginal smear showed that they were at diestrus stage with plasma low E2 levels. |
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Authors:
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Ma Eugenia Dávila-Esqueda; Ma Esther Jiménez-Capdeville; Juan Manuel Delgado; Esperanza De la Cruz; Celia Aradillas-García; Verónica Jiménez-Suárez; Reynaldo Falcón Escobedo; Joel Rodríguez Llerenas |
Publication Detail:
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Type: Journal Article Date: 2010-06-26 |
Journal Detail:
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Title: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie Volume: 64 ISSN: 1618-1433 ISO Abbreviation: Exp. Toxicol. Pathol. Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2011-12-05 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9208920 Medline TA: Exp Toxicol Pathol Country: Germany |
Other Details:
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Languages: eng Pagination: 25-30 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier GmbH. All rights reserved. |
Affiliation:
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Departamento de Farmacología, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, Av. V. Carranza 2405 "Los Filtros", 78210 San Luis Potosí, S.L.P., Mexico. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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