Document Detail


Effects of an anti-exospore monoclonal antibody on microsporidial development in vitro.
MedLine Citation:
PMID:  9881374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study we evaluated the effects of the anti-microsporidial exospore monoclonal antibody 3B6, recognizing 3 Encephalitozoon species, Encephalitozoon intestinalis (Syn. Septata intestinalis), Encephalitozoon cuniculi, and Encephalitozoon hellem on microsporidial growth in vitro. Pre-treatment of spores for 24 h with mAb 3B6 resulted in 21-29% fewer infected host cells 4 days after inoculation of the cultures compared to cultures pre-treated with medium or an irrelevant isotype control mAb (P < 0.001). Fewer intracellular spores (1.2 +/- 0.2) in infected cells were found when mAb 3B6 was present in cultures compared to cultures with medium alone (4.3 +/- 0.8) or an irrelevant isotype control mAb (4.2 +/- 0.9; P < 0.001). This decrease appeared not to be dependent on time of exposure, mAb concentration, or presence of complement. It is concluded that antibodies, particularly those directed to potential neutralizing-sensitive epitopes on spores, may have a role in the control of microsporidial growth in vitro.
Authors:
F J Enriquez; G Wagner; M Fragoso; O Ditrich
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Parasitology     Volume:  117 ( Pt 6)     ISSN:  0031-1820     ISO Abbreviation:  Parasitology     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-03-12     Completed Date:  1999-03-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  515-20     Citation Subset:  IM    
Affiliation:
Department of Veterinary Science and Microbiology, University of Arizona, Tucson 85721, USA. fje@u.arizona.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal / pharmacology*
Antibody Specificity
Cercopithecus aethiops
Complement System Proteins / pharmacology
Encephalitozoon / drug effects*,  growth & development,  immunology
Epitopes / drug effects
Fluorescent Antibody Technique
Humans
Male
Mice
Mice, Inbred BALB C
Spores / drug effects
Time Factors
Vero Cells / parasitology
Grant Support
ID/Acronym/Agency:
T37-TW00036/TW/FIC NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Epitopes; 9007-36-7/Complement System Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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