Document Detail

Effects of antenatal endotoxin and glucocorticoids on the lungs of preterm lambs.
MedLine Citation:
PMID:  10694344     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: We hypothesized that the proinflammatory response to intra-amniotic endotoxin would induce lung maturation in preterm lambs. STUDY DESIGN: Ewes were randomly assigned to receive 20 mg Escherichia coli endotoxin by intra-amniotic injection, maternal betamethasone (0.5 mg/kg), or sodium chloride solution. Preterm lambs were delivered at 125 days' gestation and underwent ventilation to assess lung function. Lung gas volume, surfactant concentrations, and inflammation were subsequently evaluated, with data analyzed by analysis of variance. RESULTS: Fetal endotoxin exposure 6 days before delivery increased compliance by 59%, increased lung gas volume 2.3-fold, increased concentrations of surfactant lipids, increased surfactant A and B protein levels, and increased messenger ribonucleic acid expressions for surfactant proteins (all P <.01, vs control group). Betamethasone exposure resulted in less consistent effects. White blood cell counts were increased in fetal membranes and lungs after endotoxin exposure, but there was no severe inflammation. CONCLUSION: A single fetal exposure to endotoxin resulted in large improvements in postnatal lung function and increases in surfactant concentrations after preterm delivery. These effects were qualitatively larger than those achieved with betamethasone.
A H Jobe; J P Newnham; K E Willet; P Sly; M G Ervin; C Bachurski; F Possmayer; M Hallman; M Ikegami
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of obstetrics and gynecology     Volume:  182     ISSN:  0002-9378     ISO Abbreviation:  Am. J. Obstet. Gynecol.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-03-21     Completed Date:  2000-03-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370476     Medline TA:  Am J Obstet Gynecol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  401-8     Citation Subset:  AIM; IM    
Division of Pulmonary Biology, Children's Hospital Medical Center, Cincinnati, Ohio 45229-3039, USA.
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MeSH Terms
Animals, Newborn
Betamethasone / pharmacology*,  therapeutic use
Bronchoalveolar Lavage Fluid
Cesarean Section
DNA Primers / chemistry
Endotoxins / administration & dosage,  pharmacology*,  therapeutic use
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Glucocorticoids / pharmacology*,  therapeutic use
Leukocyte Count
Lung / drug effects,  embryology*,  pathology
Nucleic Acid Hybridization
Proteolipids / analysis
Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactants / analysis
RNA, Messenger / chemistry,  isolation & purification
Random Allocation
Respiration, Artificial
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
Reg. No./Substance:
0/DNA Primers; 0/Endotoxins; 0/Glucocorticoids; 0/Proteolipids; 0/Pulmonary Surfactant-Associated Proteins; 0/Pulmonary Surfactants; 0/RNA, Messenger; 378-44-9/Betamethasone
Comment In:
Am J Obstet Gynecol. 2001 Jun;184(7):1584-5   [PMID:  11408890 ]

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