| Effects of the anion channel blocker DIDS on ouabain- and high K(+)-induced release of amino acids from the rat cerebral cortex. | |
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MedLine Citation:
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PMID: 10779702 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Amino acid release from the rat cerebral cortex was analyzed using an in vivo cortical cup perfusion model. Topical applications of ouabain or high extracellular K(+) were used to mimic two dimensions of ischemic conditions which promote cell swelling and amino acid release. Ouabain (30 microM) induced significant releases of taurine, gamma-aminobutyric acid (GABA), aspartate, glutamate and phosphoethanolamine. The anion channel blocker, 4, 4'-diisothiocyanatostilbene-2, 2'-disulfonic acid (DIDS; 1 mM), inhibited ouabain-induced release of all these amino acids except for glutamate. Exposure to high extracellular K(+) (75 mM) induced a delayed rise in the levels of taurine in the superfusates and an immediate increase in GABA levels. There were no significant releases of other amino acids. The release of taurine and GABA was sensitive to the blocking of anion channels with DIDS. Both ouabain- and high K(+)-induced taurine release is likely to be mediated by DIDS sensitive anion channels. The extracellular accumulation of the other amino acids, where insensitive to DIDS, may be mediated by mechanisms other than swelling-induced anion channels. |
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Authors:
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A Y Estevez; D Song; J W Phillis; M H O'Regan |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research bulletin Volume: 52 ISSN: 0361-9230 ISO Abbreviation: Brain Res. Bull. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-06-20 Completed Date: 2000-06-20 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7605818 Medline TA: Brain Res Bull Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 45-50 Citation Subset: IM |
Affiliation:
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Department of Physiology, Wayne State University School of Medicine, Detroit, MI 48201, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
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pharmacology* Amino Acids / metabolism* Animals Anions / metabolism Cerebral Cortex / drug effects, metabolism* Ion Channels / antagonists & inhibitors Male Osmolar Concentration Ouabain / pharmacology* Potassium Chloride / pharmacology* Rats Rats, Sprague-Dawley gamma-Aminobutyric Acid / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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NS26912/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Anions; 0/Ion Channels; 53005-05-3/4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 56-12-2/gamma-Aminobutyric Acid; 630-60-4/Ouabain; 7447-40-7/Potassium Chloride |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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