| Effects of the angiotensin-converting enzyme inhibitor perindopril on endothelial injury and hemostasis in rabbit endotoxic shock. | |
| | |
MedLine Citation:
|
PMID: 15024568 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
OBJECTIVE: To assess the effects of the angiotensin-converting enzyme (ACE) inhibitor (ACEI) perindopril on prolonged endothelial cell dysfunction in a rabbit endotoxic model. DESIGN: Randomized, controlled, interventional trial. SETTING: University animal laboratory. SUBJECTS: A total of 65 male New Zealand White rabbits, randomly assigned to one of eight groups. INTERVENTIONS: Endotoxic shock was induced by a single lipopolysaccharide (LPS, serotype O55:B5) bolus (0.5 mg.kg(-1), i.v., Escherichia coli endotoxin). Coagulation factors and expression of monocyte tissue factor (TF) were determined by functional assay. Endothelium-dependent vascular relaxation was assessed by in vitro vascular reactivity. Immunohistochemical staining (CD31) was performed to assess endothelial injury of the abdominal aorta. These parameters were studied 5 days (D5) after the onset of endotoxic shock. Rabbits were randomized to receive perindopril (1 mg kg(-1) day(-1) orally) alone, or with N(G)-nitro-L-arginine methyl ester (L-NAME; 15 mg kg(-1) day(-1) orally), or L-NAME alone initiated 7 days before the onset of endotoxic shock and maintained for 5 days afterward. MEASUREMENTS AND RESULTS: Perindopril prevented altered endothelium-dependent relaxation to acetylcholine induced by LPS injection (E(max)=75.6+/-3.7 vs 42.3+/-9.4% in LPS group, p<0.05). This effect was inhibited by co-treatment with L-NAME. Perindopril had no effect on either LPS-induced endothelial histological injury or monocyte TF expression. CONCLUSION: These data suggest that perindopril can prevent endothelial dysfunction in endotoxin-induced shock through an NO-dependent mechanism. |
| | |
Authors:
|
Eric Wiel; Qian Pu; Jérôme Leclerc; Delphine Corseaux; Régis Bordet; Niels Lund; Brigitte Jude; Benoît Vallet |
Related Documents
:
|
8020638 - Effect of superoxide dismutase on endothelium-dependent relaxation of aorta from endoto... 15364008 - 15d-prostaglandin j2 reduces multiple organ failure caused by wall-fragment of gram-pos... 9049158 - Sympathetic nervous responses during cytokine-induced fever in conscious rabbits. 12107688 - Increased endogenous carbon monoxide production in severe sepsis. 2076728 - Yohimbine bioavailability in humans. 9077718 - The effects of oral doses of lansoprazole and omeprazole on gastric ph. |
Publication Detail:
|
Type: Journal Article Date: 2004-03-13 |
Journal Detail:
|
Title: Intensive care medicine Volume: 30 ISSN: 0342-4642 ISO Abbreviation: Intensive Care Med Publication Date: 2004 Aug |
Date Detail:
|
Created Date: 2004-08-04 Completed Date: 2004-12-14 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 7704851 Medline TA: Intensive Care Med Country: United States |
Other Details:
|
Languages: eng Pagination: 1652-9 Citation Subset: IM |
Affiliation:
|
Department of Anesthesiology, Lille University Hospital, Lille, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Analysis of Variance Angiotensin-Converting Enzyme Inhibitors / pharmacology* Animals Blood Gas Analysis Disease Models, Animal Endothelium, Vascular / drug effects*, physiopathology Hemostasis / drug effects* Immunohistochemistry NG-Nitroarginine Methyl Ester / pharmacology Perindopril / pharmacology* Rabbits Regression Analysis Shock, Septic / chemically induced, physiopathology* Statistics, Nonparametric |
| Chemical | |
Reg. No./Substance:
|
0/Angiotensin-Converting Enzyme Inhibitors; 50903-99-6/NG-Nitroarginine Methyl Ester; 82834-16-0/Perindopril |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Identifying the potential organ donor: an audit of hospital deaths.
Next Document: Transaminase levels in ventilated children with respiratory syncytial virus bronchiolitis.