Document Detail


Effects of altered gravity on the actin and microtubule cytoskeleton of human SH-SY5Y neuroblastoma cells.
MedLine Citation:
PMID:  17180506     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human SH-SY5Y neuroblastoma cells were used to study the effects of altered gravity on the actin and microtubule cytoskeleton dynamics. A cholinergic stimulation of the cells during a 6 min period of changing gravity (3 parabolas) resulted in an enhanced actin-driven protrusion of evoked lamellipodia. Likewise, the spontaneous protrusive activity of nonactivated cells was promoted during exposure to changing gravity (6 up to 31 parabolas). Ground-based experiments revealed a similar enhancement of the spontaneous and evoked lamellar protrusive activity when the cells were kept at 2 g hypergravity for at least 6 min. This gravity response was independent of the direction of the acceleration vector in respect to the cells. Exposure of the cells to "simulated weightlessness" (clinorotation) had no obvious influence on this type of lamellar actin cytoskeleton dynamics. A 20 min exposure of the cells to simulated weightlessness or to changing gravity (6 to 31 parabolas) - but not to 2 g (hypergravity, centrifugation) - resulted in an altered arrangement of microtubules indicated by bending, turning, and loop formation. A similar altered arrangement was shown by microtubules which had polymerized into lamellipodia after release from a taxol block at simulated weightlessness (clinorotation) or during changing gravity (5 parabolas). Our data suggest that in human SH-SY5Y neuroblastoma cells, microgravity affects the dynamics and spatial arrangement of microtubules but has no influence on the Rac-controlled lamellar actin cytoskeleton dynamics and cell spreading. The latter, however, seems to be promoted at hypergravity.
Authors:
H Rösner; T Wassermann; W Möller; W Hanke
Related Documents :
3396056 - An ultrastructural study of mitosis and cytokinesis in normal 'resting' human breast.
12006626 - The plant spc98p homologue colocalizes with gamma-tubulin at microtubule nucleation sit...
12489696 - Centrosome separation: respective role of microtubules and actin filaments.
1538786 - Gamma-tubulin is a centrosomal protein required for cell cycle-dependent microtubule nu...
1254926 - Slit-scan cytofluorometry. basis for automated prescreening of urinary tract cytology.
6163546 - Reversible (g0) and nonreadily reversible (q) noncycling cells in human peripheral bloo...
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-12-16
Journal Detail:
Title:  Protoplasma     Volume:  229     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2006 Dec 
Date Detail:
Created Date:  2006-12-20     Completed Date:  2007-04-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  225-34     Citation Subset:  IM    
Affiliation:
Cell and Developmental Neurobiology, Institute of Zoology, University of Hohenheim, Stuttgart, Federal Republic of Germany. roesnerh@uni-hohenheim.de
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology
Cell Line, Tumor
Centrifugation
Cholinergic Agonists / pharmacology
Cytoskeleton / drug effects,  metabolism*
Gravity Sensing
Gravity, Altered*
Humans
Hypergravity
Microfilaments / drug effects,  metabolism*
Microtubules / drug effects,  metabolism*
Neuroblastoma
Neurons / drug effects,  metabolism*
Pseudopodia / metabolism
Rotation
Space Flight*
Time Factors
Weightlessness
Weightlessness Simulation
Chemical
Reg. No./Substance:
0/Cholinergic Agonists; 51-84-3/Acetylcholine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Respiratory burst as a biomarker for stress responses.
Next Document:  Microgravity dependence of excitable biological and physicochemical media.