Document Detail

Effects of alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor or ornithine decarboxylase, on testosterone-induced regeneration of prostate and seminal vesicle in castrated rats.
MedLine Citation:
PMID:  486129     Owner:  NLM     Status:  MEDLINE    
1. Castration of adult rats markedly decreases the amounts of polyamines (putrescine, spermidine and spermine) and of RNA and DNA in the ventral prostate and the seminal vesicle. 2. Daily injections of testosterone propionate to rats castrated 7 days previously increase polyamine and nucleic acid contents more rapidly in the seminal vesicle than in the ventral prostate. 3. After 7 days of androgen treatment, polyamine and nucleic acid contents of the seminal vesicle are significantly higher than those of intact animals. Nucleic acid, but not polyamine, contents return to normal values during the next 4 days of continued treatment. In the prostate, androgen treatment increases polyamine and nucleic acid contents to, but not above, normal values. 4. Repeated doses of alpha-difluoromethylornithine, a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase, totally blocked the testosterone-induced increase of putrescine and spermidine in the ventral prostate and of putrescine in the seminal vesicle. They slowed significantly the accumulation of spermine in the ventral prostate and of spermidine in the seminal vesicle. alpha-Difluoromethylornithine also retarded the testosterone-induced accumulation of RNA in the ventral prostate. However, no clear correlation was apparent between accumulation of polyamines and of nucleic acids in the two organs. 5. alpha-Difluoromethylornithine markedly slows the testosterone-induced weight gain of the prostate, but not of the seminal vesicle. Cytological studies suggest that this effect on the prostate is due to inhibition of the androgen-induced restoration of the secretion content of prostatic acini.
C Danzin; M J Jung; N Claverie; J Grove; A Sjoerdsma; J Koch-Weser
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  180     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1979 Jun 
Date Detail:
Created Date:  1979-11-21     Completed Date:  1979-11-21     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  507-13     Citation Subset:  IM    
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MeSH Terms
Nucleic Acids / metabolism
Organ Size / drug effects
Ornithine / analogs & derivatives*,  pharmacology
Ornithine Decarboxylase / antagonists & inhibitors
Polyamines / metabolism
Prostate / drug effects,  metabolism,  physiology*
Regeneration / drug effects*
Seminal Vesicles / drug effects,  metabolism,  physiology*
Testosterone / pharmacology
Reg. No./Substance:
0/Nucleic Acids; 0/Polyamines; 58-22-0/Testosterone; 7006-33-9/Ornithine; EC Decarboxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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