| Effects of the alpha-D-anomer of 5-aza-2'-deoxycytidine on L1210 mouse leukemic cells in vitro and in vivo. | |
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MedLine Citation:
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PMID: 6207913 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The alpha-D-anomer of 5-aza-2'-deoxycytidine inhibited cell growth in vitro in L1210 mouse leukemic cells (concentration causing 50% inhibition about 1 X 10(-6) M) and was also active in vivo in increasing the life span of mice with L1210 leukemia by 100% after a single i.p. injection (800 to 1000 mg/kg). This effect could be reversed by 2'-deoxycytidine. The alpha-D-anomer produced approximately 100-fold less host toxicity than did 5-aza-2'-deoxycytidine. It was observed that alpha-D-anomer, when kept at 23 degrees for 72 hr, inhibited the uptake of radioactive 2'-deoxycytidine by L1210 cells in vitro as well as the phosphorylation of 2'-deoxycytidine by a cell-free extract from L1210 cells. These effects could not be obtained with the fresh solution of the drug. It is proposed that the action of alpha-D-anomer is due to its conversion to 5-aza-2'-deoxycytidine as shown by high-performance liquid chromatography performed on water solutions of both anomers. |
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Authors:
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J Veselý; A Pískala |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cancer research Volume: 44 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1984 Nov |
Date Detail:
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Created Date: 1984-11-28 Completed Date: 1984-11-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5165-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Azacitidine / analogs & derivatives*, therapeutic use, toxicity Cell Division / drug effects DNA Replication / drug effects Deoxycytidine Kinase / metabolism Drug Evaluation, Preclinical Isomerism Leukemia L1210 / drug therapy*, pathology Mice Transcription, Genetic / drug effects |
| Chemical | |
Reg. No./Substance:
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2353-33-5/decitabine; 320-67-2/Azacitidine; EC 2.7.1.74/Deoxycytidine Kinase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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