Document Detail

Effects of age on morphology, protein synthesis and secretagogue-evoked secretory responses in the rat lacrimal gland.
MedLine Citation:
PMID:  12870649     Owner:  NLM     Status:  MEDLINE    
This study investigated changes in the morphology and protein synthesis and protein and peroxidase secretion due to peptidergic and aminergic stimulation from rat lacrimal gland acinar cells of 3-5, 9, 12, 20 and 24 month old rats. There was a marked reduction in the presence of Golgi apparatus in the acinar cells of glands from the 24 month old rats coupled to dilatation and degeneration of rough endoplasmic reticulum, when compared to that in the acinar cells of glands from 3-5 and 12 month old rats. Following incorporation of tritiated leucine for 360 min (6 h), the amount of newly synthesised protein in acinar cells of the 12 month old rats was significantly (p < 0.01) higher than that in the acinar cells of 3-5 month old animals. However, at 20 months the amount of newly synthesised protein in these acinar cells was significantly (p < 0.01) reduced to less than that in acinar cells of both the 3-5 and 12 month old animals. Immunohistochemical and immunofluorescence studies identified the presence of substance P (SP), vasoactive intestinal peptide (VIP), histamine and 5-hydroxytryptamine (5-HT) in the lacrimal glands of 3-5 month old rats. Stimulation by either SP, VIP, histamine or 5-HT resulted in significant increases in total protein output and peroxidase release from acinar cells of the 3-5 month old rats. However, all responses to the secretagogues were reduced with ageing from 3-5 to 24 months of age. The results indicate that ageing is associated with alteration in the ability of acinar cells to synthesise and secrete proteins.
Clare E Draper; Jaipaul Singh; Ernest Adeghate
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  248     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-07-21     Completed Date:  2004-04-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  7-16     Citation Subset:  IM    
Department of Biological Sciences, University of Central Lancashire, Preston, UK.
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MeSH Terms
Age Factors
Cytoplasm / metabolism
Endoplasmic Reticulum, Rough / metabolism
Golgi Apparatus / metabolism
Histamine / biosynthesis,  pharmacology
Lacrimal Apparatus / metabolism*
Leucine / chemistry,  metabolism
Microscopy, Electron
Microscopy, Fluorescence
Peroxidases / metabolism
Protein Biosynthesis
Rats, Sprague-Dawley
Serotonin / biosynthesis,  pharmacology
Substance P / biosynthesis,  pharmacology
Time Factors
Vasoactive Intestinal Peptide / metabolism,  pharmacology
Reg. No./Substance:
33507-63-0/Substance P; 37221-79-7/Vasoactive Intestinal Peptide; 50-67-9/Serotonin; 51-45-6/Histamine; 61-90-5/Leucine; EC 1.11.1.-/Peroxidases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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