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Effects of aerosol formulation to amino acids and fatty acids contents in Haruan extract.
MedLine Citation:
PMID:  22186302     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Haruan (Channa striatus) extract was formulated to aerosol for wound and burn treatment. Haruan extract is containing amino acids and fatty acids that important for wound healing process. The purpose of this study is to observe the effect of formulation and other excipients in the formula to amino acids and fatty acids content in Haruan extract before and after formulated into aerosol. Precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) method is used for amino acids analysis. Fatty acids in Haruan extract were esterified using transesterification method to form FAMEs before analyzed using GC. Boron trifluoride-methanol reagent is used for transesterification. Tyrosine and methionine concentrations were different after formulated. The concentrations were decrease. There are six fatty acids have amount that significantly different after formulated into concentrate and aerosol. Contents of these fatty acids were increase. Generally, fatty acids which had content increased after formulated were the long-chain fatty acids. This might be happen because of chain extension process. Saponification and decarboxylation would give the chain extended product. Therefore contents of long-chain fatty acids were increase. Generally, the aerosol formulation did not affect the amino acids concentrations in Haruan extract while some long-chain fatty acids concentrations were increase after formulated into concentrate and aerosol.
Authors:
- Febriyenti; Saringat Bin Baiabaie; Lia Laila
Publication Detail:
Type:  JOURNAL ARTICLE    
Journal Detail:
Title:  Pakistan journal of pharmaceutical sciences     Volume:  25     ISSN:  1011-601X     ISO Abbreviation:  -     Publication Date:  2012 Jan 
Date Detail:
Created Date:  2011-12-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9426356     Medline TA:  Pak J Pharm Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  1-6     Citation Subset:  -    
Affiliation:
Faculty of Pharmacy, Andalas University, Padang, Indonesia.
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