Document Detail


Effects of advanced selection methods on sperm quality and ART outcome: a systematic review.
MedLine Citation:
PMID:  21873262     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Current routine semen preparation techniques do not inclusively target all intrinsic sperm characteristics that may impact the fertilization potential. In order to address these characteristics, several methods have been recently developed and applied to sperm selection. The objective of this study was to systematically review the literature describing these advanced sperm selection methods focusing on their anticipated benefits on sperm quality and assisted reproductive technique (ART) outcome.
METHODS: Systematic literature review was conducted by means of a Medline literature search. Sperm quality parameters assessed included: motility, morphology, viability, DNA integrity, apoptosis and maturity. ART outcomes assessed included: fertilization, embryo quality, pregnancy, abortion and live birth rates.
RESULTS: A total of 44 studies were identified describing four advanced sperm selection methods based on: (i) surface charge (electrophoresis and zeta potential), (ii) apoptosis (magnetic cell sorting and glass wool), (iii) membrane maturity (hyaluronic acid binding) and (iv) ultramorphology (high magnification). Selection of high-quality sperm including improvements in DNA integrity, resulted from the application of these methods. Fertilization and pregnancy rates showed improvement following some of the advanced sperm selection techniques.
CONCLUSIONS: While some of the advanced sperm selection methods are of value in specific clinical ART settings, others are in need of further evaluation. More clinical studies on safety and efficacy are needed before the implementation of advanced sperm selection methods could be universally recommended in ART.
Authors:
Tamer M Said; Jolande A Land
Publication Detail:
Type:  Journal Article; Review     Date:  2011-08-25
Journal Detail:
Title:  Human reproduction update     Volume:  17     ISSN:  1460-2369     ISO Abbreviation:  Hum. Reprod. Update     Publication Date:    2011 Nov-Dec
Date Detail:
Created Date:  2011-10-13     Completed Date:  2012-02-03     Revised Date:  2012-05-18    
Medline Journal Info:
Nlm Unique ID:  9507614     Medline TA:  Hum Reprod Update     Country:  England    
Other Details:
Languages:  eng     Pagination:  719-33     Citation Subset:  IM    
Affiliation:
Andrology Laboratory and Reproductive Tissue Bank, The Toronto Institute for Reproductive Medicine (ReproMed), 56 Aberfoyle Crescent, Toronto, ON, Canada M8X 2W4. tsaid@repromed.ca
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MeSH Terms
Descriptor/Qualifier:
Cell Separation
Female
Humans
Infant, Newborn
Male
Pregnancy
Pregnancy Outcome
Reproductive Techniques, Assisted*
Sperm Motility
Spermatozoa / cytology,  physiology*
Treatment Outcome
Comments/Corrections
Comment In:
J Urol. 2012 May;187(5):1794   [PMID:  22494753 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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