Document Detail

Effects of adipose-derived mesenchymal cells on ischemia-reperfusion injury in kidney.
MedLine Citation:
PMID:  22398959     Owner:  NLM     Status:  Publisher    
BACKGROUND: Acute kidney injury (AKI) is a critical condition for kidney and other remote organs, including the lung. However, available treatments for AKI are limited. In this study, we explored the effect of adipose-derived mesenchymal cells on a mouse model of AKI. METHODS: Adipose-derived mesenchymal cells were isolated from mouse subcutaneous and peritoneal adipose tissue by digestion with collagenase type I. The left renal artery and vein of C57BL/6 mice were clamped for 45 min to induce ischemia and were injected with the adipose-derived mesenchymal cells [1 × 10(5) cells/0.2 ml phosphate-buffered saline (PBS)] or 0.2 ml PBS via the tail vein on days 0, 1, and 2. RESULTS: The adipose-derived mesenchymal cells had stem-cell surface markers and multilineage differentiating potentials. Administered adipose-derived mesenchymal cells homed primarily into lung. Interestingly, repeated administration of adipose-derived mesenchymal cells reduced acute tubular necrosis and interstitial macrophage infiltration in the injured kidney, accompanied with reduced cytokine and chemokine expression. CONCLUSION: Adipose-derived mesenchymal cells can be used as cell-based therapy for ischemic kidney injury.
Kengo Furuichi; Hidemi Shintani; Yoshio Sakai; Takahiro Ochiya; Kouji Matsushima; Shuichi Kaneko; Takashi Wada
Related Documents :
18357619 - Myosin-va mediates rna distribution in primary fibroblasts from multiple organs.
14671129 - Hepatitis c virus subgenomic replicons in the human embryonic kidney 293 cell line.
21274459 - Antimetastatic potentials of dioscorea nipponica on melanoma in vitro and in vivo.
21179029 - Attenuated reovirus displays oncolysis with reduced host toxicity.
21290459 - (1) h-mrs can detect aberrant glycosylation in tumour cells: a study of the hela cell l...
15496479 - Mek1 restores migration of polyamine-depleted cells by retention and activation of rac1...
1343849 - Stimulation of amino acid efflux from cells by extracellular serine.
25316819 - The novel organic arsenical darinaparsin induces mapk-mediated and shp1-dependent cell ...
1445669 - Phytochemicals potentiate interleukin-2 generated lymphokine-activated killer cell cyto...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-3-8
Journal Detail:
Title:  Clinical and experimental nephrology     Volume:  -     ISSN:  1437-7799     ISO Abbreviation:  -     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-3-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9709923     Medline TA:  Clin Exp Nephrol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Division of Blood Purification, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, 920-8641, Japan,
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparison of (11)C-PiB and (18)F-florbetaben for A? imaging in ageing and Alzheimer's disease.
Next Document:  Endoscopic pyloric balloon dilatation obviates the need for pyloroplasty at esophagectomy.