Document Detail

Effects of adenosine 3':5'-cyclic monophosphate and serum on synthesis of hyaluronic acid in confluent rat fibroblasts.
MedLine Citation:
PMID:  194579     Owner:  NLM     Status:  MEDLINE    
A small amount of hyaluronic acid is synthesized in confluent cultures of rat fibroblasts, which have a high content of cyclic AMP. Addition of calf serum caused a rapid decrease in the cellular cyclic AMP content and large increases in hyaluronic acid synthetase activity and hyaluronic acid production. Addition of cyclic AMP also caused a marked increase in hyaluronic acid synthetase activity within 2h and then increased hyaluronic acid production. The effects of cyclic AMP and serum on hyaluronic acid synthesis were additive. Prostaglandin E2, which increased the cyclic AMP by stimulating adenylate cyclase, was as effective as cyclic AMP in increasing hyaluronic acid synthetase activity, but AMP was far less effective than cyclic AMP. These results indicate that cyclic AMP itself stimulates the mucopolysaccharide synthesis and that the effect of serum is not due to a decrease in cyclic AMP in the cells.
M Tomida; H Koyama; T Ono
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  162     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1977 Mar 
Date Detail:
Created Date:  1977-07-18     Completed Date:  1977-07-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  539-43     Citation Subset:  IM    
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MeSH Terms
Cells, Cultured
Cyclic AMP / analysis,  pharmacology*
Glucosamine / metabolism
Glucuronosyltransferase / metabolism
Glycosaminoglycans / biosynthesis
Hyaluronic Acid / biosynthesis*
Reg. No./Substance:
0/Glycosaminoglycans; 3416-24-8/Glucosamine; 60-92-4/Cyclic AMP; 9004-61-9/Hyaluronic Acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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