Document Detail

Effects of the acyl chain composition of phosphatidylcholines on the stability of freeze-dried small liposomes in the presence of maltose.
MedLine Citation:
PMID:  11687225     Owner:  NLM     Status:  MEDLINE    
The effects of the acyl chain composition of phosphatidylcholines (PCs) on the stability of small unilamellar vesicles during freeze-drying and rehydration in the presence of maltose were studied by monitoring the retention of a trapped marker, calcein, in the internal liposome compartment. In dipalmitoyl PC, beta-oleoyl-gamma-palmitoyl-PC and egg yolk PC liposomes, good or fair retentions (>50%) were observed in the presence of maltose, but maltose was ineffective in preserving retention in the dioleoyl PC (DOPC) liposomes (<10%). The extremely low retention in the DOPC liposome was ascribed to neither a formation of the inverted hexagonal phase of the liposomal membrane nor the fusion/aggregation of the liposomes in the drying-rehydration process. Differential scanning calorimetry measurements suggested that interactions of maltose with PC headgroups were essential to stabilizing the dry liposomes. These interactions were significant in the saturated or mixed chain liposomes but were markedly reduced in the DOPC liposomes.
H Komatsu; H Saito; S Okada; M Tanaka; M Egashira; T Handa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemistry and physics of lipids     Volume:  113     ISSN:  0009-3084     ISO Abbreviation:  Chem. Phys. Lipids     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-05     Completed Date:  2002-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0067206     Medline TA:  Chem Phys Lipids     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  29-39     Citation Subset:  IM    
Division of Drugs, National Institute of Health Sciences, Osaka, 540-0005, Japan.
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MeSH Terms
Calorimetry, Differential Scanning
Drug Interactions
Fatty Acids / analysis*,  pharmacology
Fluoresceins / pharmacokinetics
Fluorescent Dyes / pharmacokinetics
Freeze Drying / methods,  standards
Liposomes / chemistry*
Maltose / metabolism,  pharmacology*
Membrane Lipids / metabolism
Nuclear Magnetic Resonance, Biomolecular
Particle Size
Phosphatidylcholines / chemistry*,  metabolism
Phosphorus Isotopes
Water / metabolism
Reg. No./Substance:
0/Fatty Acids; 0/Fluoresceins; 0/Fluorescent Dyes; 0/Liposomes; 0/Membrane Lipids; 0/Phosphatidylcholines; 0/Phosphorus Isotopes; 1461-15-0/fluorexon; 69-79-4/Maltose; 7732-18-5/Water

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