Document Detail


Effects of acute exercise and long-term exercise on total Na+,K+ -ATPase content and Na+,K+ -ATPase isoform expression profile in equine muscle.
MedLine Citation:
PMID:  19566475     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To investigate the effects of acute exercise and long-term training on Na(+),K(+)-ATPase content, mRNA isoforms, and protein concentration in equine muscle. ANIMALS: 6 Standardbreds. PROCEDURES: Horses performed a bout of exercise on a treadmill before and after 18 weeks of combined interval and endurance training. Muscle biopsy specimens were obtained from vastus lateralis muscle (VLM) and pectoralis descendens muscle (PDM) before and after exercise. The Na(+),K(+)-ATPase content, mRNA isoforms, and protein concentrations were determined by use of [(3)H]ouabain binding, real-time PCR assay, and western blotting, respectively. RESULTS: 6 Na(+),K(+)-ATPase mRNA isoforms were present in equine muscle, but only A2 and B1 proteins were detected. Exercise before training resulted in increases of mRNA isoforms A1, A2, A3, and B2 in VLM and A1 and B3 in PDM. Training increased resting values for mRNA isoforms A3 and B1 in VLM and B3 in PDM. The Na(+),K(+)-ATPase, [(3)H]ouabain binding, and proteins of mRNA A2 and B1 increased in VLM, whereas in PDM, only A2 protein increased as a result of training. After training, effects of strenuous exercise on mRNA expression were no longer detectable. CONCLUSIONS AND CLINICAL RELEVANCE: Equine muscle contained all Na(+),K(+)-ATPase mRNA isoforms, but only A2 and B1 proteins could be detected. Expression of these isoforms changed as a result of strenuous exercise and long-term training, representing an adaptive response. Determination of Na(+),K(+)-ATPase gene expression may be relevant for understanding alterations in excitability during neuromuscular diseases.
Authors:
Maarten M M van den Burg; Karin Eizema; Ellen de Graaf-Roelfsema; Eric van Breda; Inge D Wijnberg; Johannes H van der Kolk; Maria E Everts
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of veterinary research     Volume:  70     ISSN:  0002-9645     ISO Abbreviation:  Am. J. Vet. Res.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-01     Completed Date:  2009-08-18     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375011     Medline TA:  Am J Vet Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  895-901     Citation Subset:  IM    
Affiliation:
Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, NL-3584 CL Utrecht, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Animals
Gene Expression Profiling / veterinary*
Gene Expression Regulation, Enzymologic / physiology*
Horses / metabolism*
Isoenzymes
Male
Muscle, Skeletal / enzymology,  metabolism
Physical Conditioning, Animal*
RNA, Messenger / genetics,  metabolism
Sodium-Potassium-Exchanging ATPase / chemistry,  genetics,  metabolism*
Time Factors
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/RNA, Messenger; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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