Document Detail

Effects of acetylcholine on thyrotropin-releasing hormone release from the rat caecum in vitro.
MedLine Citation:
PMID:  2494104     Owner:  NLM     Status:  MEDLINE    
Effects of acetylcholine on the release of thyrotropin-releasing hormone (TRH) from the rat caecum in vitro were studied. The rat caecum was incubated in medium 199 with 1.0 mg/ml of bacitracin and 100 micrograms/ml of ascorbic acid (pH 7.4) (medium). The amount of TRH release into the medium was measured by radioimmunoassay. The immunoreactive TRH (ir-TRH) release from the rat caecum was enhanced significantly in a dose-related manner with the addition of acetylcholine, but not changed with atropine. The stimulatory effect of acetylcholine on ir-TRH release from the rat caecum was blocked with an addition of atropine. Elution profile of acid-methanol-extracted rat caecum on Sephadex G-10 was identical to that of synthetic TRH. The findings suggest that the cholinergic system stimulates TRH release from the rat caecum in vitro.
T Mitsuma; Y Hirooka; K Nakata; T Nogimori
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et métabolisme     Volume:  21     ISSN:  0018-5043     ISO Abbreviation:  Horm. Metab. Res.     Publication Date:  1989 Jan 
Date Detail:
Created Date:  1989-05-05     Completed Date:  1989-05-05     Revised Date:  2009-02-19    
Medline Journal Info:
Nlm Unique ID:  0177722     Medline TA:  Horm Metab Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  8-10     Citation Subset:  IM    
Fourth Department of Internal Medicine, Aichi Medical University, Japan.
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MeSH Terms
Acetylcholine / pharmacology*
Cecum / drug effects,  secretion*
Rats, Inbred Strains
Thyrotropin-Releasing Hormone / secretion*
Reg. No./Substance:
24305-27-9/Thyrotropin-Releasing Hormone; 51-84-3/Acetylcholine

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