Document Detail


Effects of acetylcholine in the pulmonary circulation of rabbits.
MedLine Citation:
PMID:  6491979     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pulmonary and systemic vascular beds differ in their response to several vasoactive substances. Acetylcholine (ACh) is a potent systemic vasodilator; however, its effects on the pulmonary circulation are not clear. Utilizing the isolated rabbit lung preparation perfused in situ under constant flow (92 +/- 3 ml/min) with an albumin-enriched physiologic salt solution, we studied the effects of graded concentrations of ACh (10(-10) to 10(-4) M) in the recirculating perfusate on pulmonary arterial pressure (PAP). The lungs were ventilated to 15 cm of water with 97% O2-3% CO2. ACh (10(-8) to 4 X 10(-7) M) produced a dose-dependent increase in PAP which required up to 20 min to return to predrug levels. Physostigmine (10(-5) M) maintained the maximal PAP response to ACh for the duration of the experiment (up to 2 hr). Higher concentrations of ACh produced rapid increases in PAP resulting in edema. Pre- or post-treatment with the muscarinic receptor antagonist atropine (10(-5) M) completely prevented or reversed the pressor effects of ACh, respectively. Histamine receptor (H1 and H2) or angiotensin II receptor blockade had no effect on the PAP actions of ACh, however pretreatment with the prostaglandin synthesis (cyclooxygenase) inhibitor indomethacin (10(-5) M) or aspirin (10(-3) M), completely abolished the PAP response to ACh up to concentrations of 10(-4) M. In experiments in which airway pressure was allowed to vary, it rose significantly in response to increasing concentrations of ACh. ACh-induced increases in airway pressure were unaffected by pretreatment with indomethacin (10(-5) M) but were totally inhibited by pretreatment with atropine (10(-5) M).(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
J D Catravas; J J Buccafusco; H El Kashef
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of pharmacology and experimental therapeutics     Volume:  231     ISSN:  0022-3565     ISO Abbreviation:  J. Pharmacol. Exp. Ther.     Publication Date:  1984 Nov 
Date Detail:
Created Date:  1984-12-19     Completed Date:  1984-12-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376362     Medline TA:  J Pharmacol Exp Ther     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  236-41     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / pharmacology*
Animals
Aspirin / pharmacology
Atropine / pharmacology
Blood Pressure / drug effects
Indomethacin / pharmacology
Male
Prostaglandins / secretion
Pulmonary Circulation / drug effects*
Rabbits
Vasoconstriction / drug effects
Chemical
Reg. No./Substance:
0/Prostaglandins; 50-78-2/Aspirin; 51-55-8/Atropine; 51-84-3/Acetylcholine; 53-86-1/Indomethacin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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