Document Detail


Effects of acarbose on ruminal fermentation, blood metabolites and microbial profile involved in ruminal acidosis in lactating cows fed a high-carbohydrate ration.
MedLine Citation:
PMID:  20053317     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The objective was to evaluate the effects of an inhibitor of alpha-amylase and glucosidase (acarbose, Pfizer Limited, Corby, UK) on ruminal fermentation, blood metabolism and microbial profile in dairy cows in a 2x2 cross-over experiment. Eight Holstein cows fitted with rumen cannulas (milk yield, 24.3+/-2.35 kg/d, body weight, 622+/-54 kg, days in milk, 183+/-67, 5 multiparous and 3 primiparous) were used. Treatments were: control (no additive, CTR) and alpha-amylase and glucosidase inhibitor (0.75 g acarbose-premix/cow per d, AMI). Animals were given ad-libitum access to a high non-fibre carbohydrate (NFC) partial mixed ration (PMR) containing 17.6% crude protein, 28.3% neutral detergent fibre, and 46.5% NFC in the dry matter and supplementary concentrate during milking. Blood samples were taken to determine blood glucose, insulin and urea within the first hour after the morning feeding on two separate days in each period. Samples of ruminal contents were collected during 3 d in each period at 0, 4 and 8 h after feeding to determine volatile fatty acid and ammonia-N concentrations and to quantify protozoa, Streptococcus bovis and Megasphaera elsdenii. Rumen pH was recorded electronically at 22-min intervals during 6 d in each period. Results were analysed using a mixed-effects model. Cows on AMI treatment spent less time with ruminal pH <5.6 compared with cows in the CTR group (3.74 and 6.52+/-0.704 h/d, respectively). Cows in the AMI group had greater daily average pH compared with those in the CTR group (6.05 and 5.92+/-0.042, respectively). AMI animals tended (P=0.09) to have lower Str. bovis to Meg. elsdenii ratio than CTR (4.09 and 26.8+/-12.0, respectively). These results indicate that dietary supplementation with acarbose in dairy cattle fed high-production rations may be effective in reducing the time for which rumen pH is suboptimal, with no negative effects on ruminal fermentation and blood metabolites.
Authors:
Marta Blanch; Sergio Calsamiglia; Maria Devant; Alex Bach
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-07
Journal Detail:
Title:  The Journal of dairy research     Volume:  77     ISSN:  1469-7629     ISO Abbreviation:  J. Dairy Res.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-18     Completed Date:  2010-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985125R     Medline TA:  J Dairy Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  123-8     Citation Subset:  IM    
Affiliation:
Departament de Ci?ncia Animal i dels Aliments, Universitat Aut?noma de Barcelona, 08193 - Bellaterra, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acarbose / pharmacology,  therapeutic use*
Acidosis / microbiology,  prevention & control,  veterinary*
Animals
Blood Glucose / analysis
Cattle
Cattle Diseases / blood,  microbiology,  prevention & control*
Dietary Carbohydrates / administration & dosage*
Enzyme Inhibitors / pharmacology,  therapeutic use
Female
Fermentation / drug effects
Glucosidases / antagonists & inhibitors
Hydrogen-Ion Concentration
Insulin / blood
Lactation
Milk / chemistry
Rumen / chemistry,  metabolism,  microbiology*
Stomach Diseases / microbiology,  prevention & control,  veterinary*
Urea / blood
alpha-Amylases / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Dietary Carbohydrates; 0/Enzyme Inhibitors; 11061-68-0/Insulin; 56180-94-0/Acarbose; 57-13-6/Urea; EC 3.2.1.-/Glucosidases; EC 3.2.1.1/alpha-Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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