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Effects of Xin-Ji-Er-Kang formula on 2K1C-induced hypertension and cardiovascular remodeling in rats.
MedLine Citation:
PMID:  25063306     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
ETHNOPHARMACOLOGICAL RELEVANCE: Xin-Ji-Er-Kang(XJEK),a a Chinese herbal formula,is effective against hypertension induced coronary heart disease, viral myocarditis and toxic myocarditis.In this study,the effect of XJEK on cardiovasuclar system were investigated.
AIM OF THE STUDY: To test the hypothesis that Xin-Ji-Er-Kang (XJEK) has an anti-hypertensive effect mediated through attenuation of cardiac remodeling,and amelioration of vascular endothelial dysfunction and oxidative stress.
MATERIALS AND METHODS: Hypertension was induced in Wistar rats by 2 kidney 1 clip (2K1C) treatment. The hypertensive rats were then randomly assigned into four groups and treated as follows: group 1 (Sham-operated [Sh-Op] group received only drinking water), group 2 (Induced hypertensive model+no treatment), and group 3 (Induced hypertensive+a single daily oral dose of 24g·kg(-1)XJEK treatment) and group 4 (Induced hypertensive+a single oral dose of 15mgkg(-1)Fosinopril treatment). The rats in all the defined groups were respectively treated for a period of 4 weeks. Cardiovascular parameter such as systolic blood pressure (SBP) was measured weekly by using tail-cuff apparatus; left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP) and the rate of the rise in left ventricular pressure (± dp/dt max) were measured by using PowerLab 8/30 apparatus (AD Instruments, Australia) at the end of the 8th week; heart weight/body weight (HW/BW) was determined as an index of myocardial hypertrophy (MH). Hematoxylin and eosin (H&E) and Van Gieson (VG) stain were used to assess the cardio-histological changes. Colorimetric analysis was used to assay serum superoxide dismutase (SOD) activity, malondialdehyde (MDA), nitric oxide (NO), and hydroxyproline (Hyp) contents in cardiac tissue. AngiotensinⅡ (Ang II) content in serum was assessed by radioimmunoassay; tetrahydrobiopterin (BH4) content in cardiac tissue,BNP and endothelial NOS(eNOS) in serum were determined by using ELISA, and the protein expressions of c-Jun NH2-terminal kinase (JNK), P-JNK, p38, P-p38,, and NADPH oxidases -2 (Nox-2) were measured by western blot.
RESULTS: XJEK therapy could impaire the heart systolic and diastolic function, potently improve the heart weight index, inhibite the elevation of HW/BW ratio,markedly ameliorate hemodynamic indexes, vascular remodeling index. It has blunted the decrease of SOD, NO and the increase in MDA and AngⅡserum contents, myocardial cross-section area (CSA), collagen volume fraction (CVF) and perivascular circumferential collagen area (PVCA) compared to the hypertensive model group. It also reduced the serum content of Hyp while increased BH4 levels in cardiac tissue. In addition, the expressions of Nox-2, P-JNK and P-p38MAPK were all suppressed compared to the hypertensive model group.What's more, treatment with XJEK improved endothelial dysfunction (ED) manifested by promoting eNOS activities and enhancing the NO activity in serum.
CONCLUSION: The results of the present study show that XJEK attenuates 2K1C-induced hypertension in rats, which confirms our hypothesis that XJEK has an anti-hypertensive and cardiovascular remodeling effect via attenuation of cardiac remodeling and improvement of endothelial dysfunction and oxidative stress.
Authors:
Kun Guo; Chao-Zong Lan; Ting-Ting Yu; Ling-Ling Huang; Xing-Hui Wang; Chen Pan; Shan Gao
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-7-22
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  -     ISSN:  1872-7573     ISO Abbreviation:  J Ethnopharmacol     Publication Date:  2014 Jul 
Date Detail:
Created Date:  2014-7-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
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