| Effects of X-irradiation on cell-cycle progression, induction of chromosomal aberrations and cell killing in ataxia telangiectasia (AT) fibroblasts. | |
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MedLine Citation:
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PMID: 3969079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Survival, cumulative labeling indices, chromosomal aberrations and cell-cycle distribution by flow microfluorometry (FMF) were studied in fibroblasts from normal and three ataxia telangiectasia (AT) families after X-irradiation during density-inhibition of growth and immediate release by subculture to low density. Homozygotic AT (proband) fibroblasts were very hypersensitive to cell killing by X-irradiation (D0 = 40-45 rad). Fibroblasts from AT heterozygotes (parents) were minimally hypersensitive, with D0's (100-110 rad) slightly lower than those for normal fibroblasts (D0 = 120-140 rad). There were three different response groups for a G1 phase block induced by 400 rad of X-rays: (1) minimal or no G1 block was observed in AT homozygote cell strains; (2) 10-20% of the cells were blocked in G1 in normal cell strains; and (3) 50% or more of the cells were blocked in AT heterozygote strains. FMF profiles and cumulative labeling indices showed that homozygotic AT cells irradiated in plateau phase moved into the S-phase following subculture with no additional delay over non-irradiated controls. Homozygotic AT cells showed not only a 4-5 times higher frequency of X-ray-induced chromosomal aberrations than normal strains, but approximately 30% of these were of the chromatid-type. There were no differences in the frequency or type of X-ray-induced chromosomal aberrations between normal and heterozygotic AT cells. |
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Authors:
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H Nagasawa; S A Latt; M E Lalande; J B Little |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Mutation research Volume: 148 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1985 Jan-Feb |
Date Detail:
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Created Date: 1985-03-08 Completed Date: 1985-03-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 71-82 Citation Subset: IM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Ataxia Telangiectasia / genetics* Cell Cycle / radiation effects* Cell Survival / radiation effects Cells, Cultured Child Chromosome Aberrations DNA Repair Female Heterozygote Homozygote Humans Infant, Newborn Male Middle Aged X-Rays |
| Grant Support | |
ID/Acronym/Agency:
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CA 11751/CA/NCI NIH HHS; ES-00002/ES/NIEHS NIH HHS; GM-22121/33579/GM/NIGMS NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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