| Effects of Vitamin D3 on the Expression of Growth-Related Oncogene-α in THP-1 Cells and Human Primary Monocytes. | |
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MedLine Citation:
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PMID: 22251138 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Asthma and many autoimmune diseases, such as systemic lupus erythematosus, have been reported to associate with vitamin D deficiency recently. Growth-related oncogene-α (GRO-α)/CXCL1, a neutrophil-related chemokine, have an important influence on the chronic inflammation of these diseases. It is unknown whether vitamin D has regulatory effects on GRO-α expression in human monocytes. To this end, the human monocytic leukemia cell line, THP-1, and human primary monocytes were pretreated with 1α, 25-(OH)(2) D(3) , and was stimulated with lipopolysaccharide (LPS). Supernatants were collected to determine GRO-α level by ELISA. The intracellular signaling was investigated by nuclear factor (NF)-κB inhibitor, the mitogen-activated protein kinase (MAPK) inhibitors, and Western blot. In our studies, LPS-induced GRO-α was significantly enhanced in THP-1 cells, but suppressed in human primary monocytes by 1α, 25-(OH)(2) D(3) . Western blotting revealed that 1α, 25-(OH)(2) D(3) increased LPS-stimulated pp38 expression in THP-1 cells, but suppressed LPS-stimulated pMEK1/2-pERK and pJNK in human primary monocytes. In conclusion, the opposite effects of 1α, 25-(OH)(2) D(3) on GRO-α expression in THP-1 cells and human primary monocytes indicated that the data from THP-1 cells should be further confirmed by human primary monocytes. Moreover, vitamin D3 may have potentiality in treating GRO-α-related chronic inflammatory diseases, like asthma and autoimmune diseases. |
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Authors:
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Yu-Ting Kuo; Ren-Long Jan; Chang-Hung Kuo; Po-Lin Kuo; Wei-Li Wang; Ming-Yii Huang; Huan-Nan Chen; Chih-Hsing Hung |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-17 |
Journal Detail:
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Title: Journal of food science Volume: - ISSN: 1750-3841 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0014052 Medline TA: J Food Sci Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 Institute of Food Technologists® |
Affiliation:
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Authors Y.-T. Kuo and Wang are with Dept. of Emergency, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Authors C.-H. Kuo, Chen, and Hung are with Dept. of Pediatrics, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Jan is with Dept. of Pediatrics, Chi Mei Medical Center, Liouying, Taiwan. Authors C.-H. Kuo and Hung are with Graduate Inst. of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Authors C.-H. Kuo and Hung are also with Dept. of Pediatrics, Kaohsiung Municipal Ta-Tung Hospital, Kaohsiung, Taiwan. Author P.-L. Kuo is with Inst. of Clinical Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Huang is with Dept. of Radiation Oncology, Kaohsiung Medical Univ. Hospital, Kaohsiung, Taiwan. Author Huang is also with Dept. of Radiation Oncology, School of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Author Hung is also with Dept. of Pediatrics, School of Medicine, College of Medicine, Kaohsiung Medical Univ., Kaohsiung, Taiwan. Authors Y.-T. Kuo and Jan contributed equally to the manuscript. Direct inquiries to author Hung (E-mail: pedhung@gmail.com). |
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