| Effects of TGF-beta and TGF-beta-neutralizing antibody on normal skin fibroblasts and scar-derived fibroblasts. | |
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MedLine Citation:
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PMID: 12652967 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transforming growth factor-beta (TGF-beta) is known as an important cytokine for scar formation in wound healing. The purpose of the present study was to investigate the effects of TGF-beta and its neutralizing antibody on normal skin fibroblasts and scar-derived fibroblasts in culture. Endogenous TGF-beta levels were similar in all fibroblasts. Cell proliferation increased when TGF-beta 1 or beta 2 was added to the cultures, and the increase was higher and started at a lower level in the scar-derived fibroblasts (p < 0.05). The increase of fibroblasts was suppressed by the addition of TGF-beta-neutralizing antibody to the cultures, and the suppression rate was higher in the scar-derived cells (p < 0.05). Percentages of the cells in the growth phases of cell cycle decreased in the normal skin fibroblasts (p < 0.05) when TGF-beta-neutralizing antibody was added. Our findings showed that scar-derived fibroblasts and normal skin fibroblasts have a different sensitivity to TGF-beta. Further study is needed on the effect of the neutralizing antibody to the cell counts and cell cycle of scar-derived fibroblasts. |
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Authors:
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Toshihiko Yamauchi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Kurume medical journal Volume: 49 ISSN: 0023-5679 ISO Abbreviation: Kurume Med J Publication Date: 2002 |
Date Detail:
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Created Date: 2003-03-25 Completed Date: 2003-04-25 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985210R Medline TA: Kurume Med J Country: Japan |
Other Details:
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Languages: eng Pagination: 171-6 Citation Subset: IM |
Affiliation:
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Department of Plastic and Reconstruction Surgery, Kurume University School of Medicine, 67 Asahi-machi, Kurume 830-0011, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Cycle
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drug effects Cell Division / drug effects Cells, Cultured Cicatrix / pathology* Fibroblasts / cytology, drug effects Humans Neutralization Tests Skin / cytology*, drug effects* Transforming Growth Factor beta / antagonists & inhibitors, immunology, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Transforming Growth Factor beta |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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