Document Detail


Effects of Survivin on cell proliferation and apoptosis in MG-63 cells in vitro.
MedLine Citation:
PMID:  18996209     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Osteosarcoma is the most frequent primary malignant tumor of the skeleton and occurs mainly in children and adolescent. The prognosis of osteosarcoma is very poor due to its aggressive and no effective treatment. This study is the first to investigate the anti-cancer effects of antisense pEGFP-C1-Survivin on human osteosarcoma cells. It was shown in our results that Survivin blockaded could significantly induce apoptosis and inhibit the invasive of osteosarcoma cells line MG-63. The effects were probably produced by the decreased expression of Survivin induced by antisense pEGFP-C1-Survivin which was examined by RT-PCR and western blotting. All these suggested that Survivin should be very important in the development of osteosarcoma and Survivin blockaded by using antisense pEGFP-C1-Survivin could markedly inhibit the proliferation and invasion of osteosarcoma cells line MG-63, partially reversed their malignant phenotype. Targeting Survivin might be a promising option in the treatment of osteosarcoma.
Authors:
Xinjun Liang; Mingxu Da; Zhiqiang Zhuang; Weiguang Wu; Zhuang Wu; Yanfeng Wu; Huiyong Shen
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Publication Detail:
Type:  Journal Article     Date:  2008-11-01
Journal Detail:
Title:  Cell biology international     Volume:  33     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-22     Completed Date:  2009-01-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  119-24     Citation Subset:  IM    
Affiliation:
Department of Orthopaedics, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, PR China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Blotting, Western
Bone Neoplasms / pathology,  therapy*
Cell Line, Tumor
Cell Proliferation
Down-Regulation / genetics
Humans
Microtubule-Associated Proteins / antagonists & inhibitors*,  genetics,  physiology
Oligonucleotides, Antisense / metabolism*
Osteosarcoma / pathology,  therapy*
RNA Interference
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Transfection
Chemical
Reg. No./Substance:
0/BIRC5 protein, human; 0/Microtubule-Associated Proteins; 0/Oligonucleotides, Antisense; 0/RNA, Messenger

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