Document Detail


The effects of Stichopus japonicus acid mucopolysaccharide on the apoptosis of the human hepatocellular carcinoma cell line HepG2.
MedLine Citation:
PMID:  20051817     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this study, the effects of Stichopus japonicus acid mucopolysaccharide (SJAMP) on the apoptosis of the human hepatocellular carcinoma cell line HepG2 were examined. The underlying mechanism was investigated by determining the effect of SJAMP on the expression of Bcl-2 and nm23-H1 genes in HepG2 cells. In vitro cultured HepG2 cells were treated with different concentrations of SJAMP. The dimethylthiazol (MTT) assay was used to determine the inhibition of cell proliferation. Expression of Bcl-2 and nm23-H1 genes was determined by Western blot analysis. The results showed that SJAMP inhibited the proliferation of HepG2 cells in a time- and dose-dependent manner, SJAMP induced apoptosis in HepG2 cells, and SJAMP decreased the expression of Bcl-2 and increased the expression of nm23-H1. We conclude that SJAMP inhibits the proliferation of HepG2 cells by inducing apoptosis. These results provide a theoretical basis for the utilization of SJAMP as a potential antitumor component for the treatment of hepatocellular carcinoma.
Authors:
Yun Lu; Bing-Yuan Zhang; Qian Dong; Bao-Lei Wang; Xi-Bao Sun
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American journal of the medical sciences     Volume:  339     ISSN:  1538-2990     ISO Abbreviation:  Am. J. Med. Sci.     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-02-11     Completed Date:  2010-03-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0370506     Medline TA:  Am J Med Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  141-4     Citation Subset:  AIM; IM    
Affiliation:
Second Department of General Surgery, Qingdao University, Qingdao City, People's Republic of China. cloudylucn@126.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / chemistry,  pharmacology
Apoptosis / drug effects*
Carcinoma, Hepatocellular*
Cell Line, Tumor
Cell Proliferation
Gene Expression Regulation, Neoplastic / drug effects
Glycosaminoglycans / chemistry*,  pharmacology*
Humans
Liver Neoplasms
Neoplasm Proteins / genetics,  metabolism
Stichopus / chemistry*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Glycosaminoglycans; 0/Neoplasm Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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