| The effects of sample size on the outcome of ovarian tissue cryopreservation. | |
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MedLine Citation:
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PMID: 18992115 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cryopreservation of ovarian tissue is known to affect follicular survival. Several variables may be responsible for this. Little attention has focused on the effect of the size of the fragment to be cryopreserved. This study was conducted to assess the effect of the size of the tissue on follicular histology after freezing with 1,2-propanediol. Histological evaluations were performed of control and cryopreserved tissue. Fragments were cut 10 x 3 x 2 mm(3) (2 mm group) or 10 x 3 x 4 mm(3) (4 mm group). Percentages of normal follicles in control fragments cut into 2 and 4 mm slices were 56% and 34%, respectively. The relative risks to obtain normal follicles in the 2 mm and the 4 mm fragments after cryopreservation were 0.63 and 0.47, respectively. Freezing reduced follicle survival to a significantly greater extent in the larger tissue fragments. There is an increased risk of damage to primary and primordial follicles, when the tissue slices are cut with all dimensions larger than 2 mm. |
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Authors:
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M Ferreira; A Bos-Mikich; N Frantz; J L Rodrigues; A L Brunetto; G Schwartsmann |
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Publication Detail:
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Type: Journal Article Date: 2008-10-16 |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 45 ISSN: 1439-0531 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-02-08 Completed Date: 2010-04-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 99-102 Citation Subset: IM |
Affiliation:
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Centro de Pesquisa e Reprodu??o Humana Nilo Frantz, Porto Alegre, RS, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle* Cryopreservation / methods, veterinary* Female Ovarian Follicle / anatomy & histology, physiology Ovary / anatomy & histology, physiology* Tissue Survival / physiology Tissue and Organ Harvesting / methods, veterinary |
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