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Effects of Salvia miltorrhiza in neural differentiation of rat mesenchymal stem cells with optimized protocol.
MedLine Citation:
PMID:  21549822     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
AIM OF THE STUDY: The present study was aimed to explore the effects of Salvia miltorrhiza in inducing rMSCs to differentiate into functional neurons. MATERIALS AND METHODS: rMSCs were isolated and cultured in vitro, then Salvia miltorrhiza was added to induce rMSCs to differentiate repeatedly for 5 times with an optimized protocol, and neurophysiological functions such as action potential, endocytosis and exocytosis of the induced cells were investigated. RESULTS: About 98% of rMSCs expressed markers related to neural stem cells after treatment with preinduction medium, but they remained fibroform, the classical morphological state of MSCs, after exposure to induction medium for 2h, and the induced cells showed a neural shape. Next, fetal bovine serum (FBS) was added into the induction medium, transforming the neuron-like cells into fibroform cells. Finally, after exposure to induction medium, the cells could be transformed into neuron-like cells again. After the procedure was repeated 5 times, the induced cells displayed a classical neural shape and more than 95% of them expressed neural markers, including TUJ-1, NF and synaptophysin. Furthermore, the induced cells displayed neurophysiological functions, as characterized by action potential, endocytosis and exocytosis in response to a solution with a high concentration of potassium (K(+)). CONCLUSION: Salvia miltorrhiza can induce rMSCs to differentiate into neurons with neurophysiological functions efficiently by an optimized protocol.
Authors:
Linyan Hu; Jialin Yu; Fang Li; Boman Chen; Luquan Li; Guanxin Liu
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-4-28
Journal Detail:
Title:  Journal of ethnopharmacology     Volume:  -     ISSN:  1872-7573     ISO Abbreviation:  -     Publication Date:  2011 Apr 
Date Detail:
Created Date:  2011-5-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7903310     Medline TA:  J Ethnopharmacol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011. Published by Elsevier Ireland Ltd.
Affiliation:
Department of Neonatology, Children's Hospital, Chongqing Medical University, Chongqing 400014, China.
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