Document Detail

Effects of SCO-spondin thrombospondin type 1 repeats (TSR) in comparison to Reissner's fiber material on the differentiation of the B104 neuroblastoma cell line.
MedLine Citation:
PMID:  11456412     Owner:  NLM     Status:  MEDLINE    
SCO-spondin is a newly identified protein that is strongly expressed in the subcommissural organ (SCO), an ependymal differentiation of the brain. When released into the cerebrospinal fluid at the entrance to the Sylvian aqueduct, the glycoproteins condense and form a thread-like structure, Reissner's fiber (RF). To analyze the role of SCO-spondin on neuronal development, we studied the effects induced by an oligopeptide derived from a thrombospondin type 1 repeat (TSR) of SCO-spondin on neuroblastoma B104 cells and compared them with the effects of soluble RF material containing complete SCO-spondin proteins. In low density cell culture, the TSR peptide first induced a notable flattening of cells accompanied by increased neurite outgrowth. Grouping of these differentiated B104 cells, which later formed dense aggregates, was then observed with increasing time in culture. Soluble RF material induced similar morphological changes and neurite-promoting effects on B104 cells, although the cells remained evenly distributed throughout the culture time and no aggregates were visible. In high-density cell culture, both TSR peptide and RF material induced prominent neurite outgrowth and subsequent rapid cell aggregation. Whereas soluble RF material inhibited cell proliferation, no respective effect was observed in the presence of the TSR peptide. A direct interaction of TSR peptide and soluble RF material with a B104 cell binding site was revealed by increased B104 cell metabolic activity by flow cytometry.
F El-Bitar; M Bamdad; B Dastugue; A Meiniel
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Cell and tissue research     Volume:  304     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  2001 Jun 
Date Detail:
Created Date:  2001-07-17     Completed Date:  2002-01-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  361-9     Citation Subset:  IM    
INSERM U 384 et Laboratoire de Biochimie Médicale, Faculté de Médecine, Clermont-Ferrand, France.
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MeSH Terms
Amino Acid Motifs / genetics
Amino Acid Sequence / genetics
Cell Adhesion Molecules, Neuronal / chemistry*,  pharmacology*
Cell Aggregation / drug effects
Cell Differentiation / drug effects
Cell Division / drug effects
Cell Size / drug effects
Conserved Sequence / genetics
Flow Cytometry
Neurites / drug effects,  ultrastructure
Neurons / cytology,  drug effects,  metabolism
Oligopeptides / pharmacology
Repetitive Sequences, Amino Acid
Thrombospondin 1 / chemistry
Tumor Cells, Cultured
Reg. No./Substance:
0/Cell Adhesion Molecules, Neuronal; 0/Oligopeptides; 0/SCO-spondin; 0/Thrombospondin 1

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