Document Detail

Effects of RRR-alpha-tocopherol on leukocyte expression of HSP72 in response to exhaustive treadmill exercise.
MedLine Citation:
PMID:  12215965     Owner:  NLM     Status:  MEDLINE    
Previous research revealed an increased expression of HSP72 in leukocytes after vigorous endurance exercise. We questioned whether more intensive but shorter exercise also induces leukocyte HSP72 synthesis. To delineate the role of reactive oxygen species (ROS) in exercise-related HSP72 induction, we additionally examined the effect of RRR-alpha-tocopherol (alpha-toc) on HSP72 expression using a double-blind placebo (P) controlled cross-over design. After supplementation with alpha-toc (500 I.U. daily) or P for 8 days, 9 male subjects performed a combined exhaustive treadmill protocol (total duration 29.4 +/- 2.0 min). HSP72 was assessed on mRNA (RT-PCR) and protein levels (flow cytometry). HSP72 mRNA rose 3 h after exercise only in the P group, but individual differences (alpha-toc - P) did not reveal significant treatment effects. A moderate but significant rise of HSP72 protein occurred in granulocytes up to 48 h after exercise. Three hours post-exercise, granulocyte HSP72 protein was lower when subjects received alpha-toc, but this effect vanished 24 and 48 h post-exercise. Exhaustive treadmill exercise augments HSP72 mRNA in leukocytes and induced a moderate but prolonged response of granulocyte HSP72 protein. These exercise effects are lower when compared to earlier findings obtained after vigorous endurance exercise. ROS seem to be involved, but do not play the major role in the induction of granulocyte HSP72 synthesis after exhaustive exercise.
A M Niess; E Fehrenbach; E Schlotz; M Sommer; C Angres; K Tschositsch; N Battenfeld; I C Golly; H K Biesalski; H Northoff; H H Dickhuth
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of sports medicine     Volume:  23     ISSN:  0172-4622     ISO Abbreviation:  Int J Sports Med     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-09-06     Completed Date:  2003-02-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008349     Medline TA:  Int J Sports Med     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  445-52     Citation Subset:  IM    
Medical Clinic & Polyclinic, Department of Sports Medicine, Eberhard-Karls University of Tuebingen, 72074 Tuebingen, Germany.
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MeSH Terms
Exercise Tolerance / physiology*
HSP72 Heat-Shock Proteins
Heart Rate
Heat-Shock Proteins / blood*
Leukocytes / metabolism*
Reverse Transcriptase Polymerase Chain Reaction
alpha-Tocopherol / pharmacology*
Reg. No./Substance:
0/HSP72 Heat-Shock Proteins; 0/Heat-Shock Proteins; 59-02-9/alpha-Tocopherol

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