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Effects of Protein-Simulated Body Fluid Mixing Methods on Characteristics of Bone-Like Mineral.
MedLine Citation:
PMID:  23144533     Owner:  NLM     Status:  Publisher    
This study examined effects of protein mixing methods into modified simulated body fluid (mSBF) on the crystalline structure and morphology of bone-like mineral (BLM) coated on poly(lactic-co-glycolic acid) PLGA. Using bovine serum albumin (BSA) as a model protein, four sample groups were prepared: the N-BLM group was coated by soaking substrates in mSBF without BSA; the B-BLM group was coated by soaking in mSBF with BSA added immediately before soaking; the Ca-BLM group was coated by soaking in Ca-mSBF prepared by premixing BSA with a CaCl(2) solution before preparing the mSBF; the P-BLM group was coated by soaking in P-mSBF made by premixing the BSA with a KH(2)PO(4) solution. The B-BLM and Ca-BLM groups exhibited densely coated, thick BLM layers, whereas the P-BLM group exhibited loosely connected BLM clusters. The hydroxyapatite (HAp) crystallites of the B-BLM and Ca-BLM groups were aligned along the c-axis, but those of the P-BLM group were disordered and had a lower crystallinity. The alignment to the c-axis of Ca-BLM and the disordered orientation of P-BLM was caused by calcium ions bound to BSA in Ca-mSBF and phosphate ions bound to BSA in P-mSBF, respectively. These results show that the crystallinity and morphology of BLM can be controlled by the mixing of BSA in mSBF. The crystallinity of BLM is closely connected to its solubility. Therefore, the release characteristics of growth factors and cell regulation on BLM could be affected by the changes in the crystalinity of BLM.
Ho-Jun Song; Yeong-Joon Park; Won-Jin Moon; Linh N Luong; David H Kohn
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-7-27
Journal Detail:
Title:  Materials science & engineering. C, Materials for biological applications     Volume:  32     ISSN:  0928-4931     ISO Abbreviation:  Mater Sci Eng C Mater Biol Appl     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-11-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101484109     Medline TA:  Mater Sci Eng C Mater Biol Appl     Country:  -    
Other Details:
Languages:  ENG     Pagination:  2501-2507     Citation Subset:  -    
Department of Dental Materials and MRC for Biomineralization Disorders, School of Dentistry, Chonnam National University, Gwangju 500-757, Korea.
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