Document Detail

Effects of protein inhibitors and auxin on nucleic Acid metabolism in peanut cotyledons.
MedLine Citation:
PMID:  16656356     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The accumulation of labeled phosphorus into newly synthesized nucleic acids or peanut cotyledon slices incubated with chloramphenicol, puromycin, or 2,4-dichlorophenoxyacetic acid (2,4-D) was reduced. Promotion of nucleic acid synthesis was not noted by any of these chemicals. Chloramphenicol completely inhibited the synthesis of the DNA-RNA fraction at 1.25 x 10(-3)m while soluble and ribosomal RNA was inhibited by 70% and 80%, respectively. At the same concentration messenger RNA was inhibited by only 40%. These effects suggest that chloramphenicol inhibit nucleic acid synthesis in peanut cotyledons in a differential manner. Similar results were noted for DNA at low concentrations of 2,4-D. However, at high concentrations of 2,4-D, DNA as well as RNA fractions were inhibited in a similar manner at a given concentration. Puromycin did not differentially inhibit nucleic acid synthesis except at 2 x 10(-3)m where DNA was least inhibited.Nondifferential inhibition suggests that a common site or precursor pool essential for the synthesis of all nucleic acid fractions is altered. Differential inhibition may be due to the interference with a specific rate-limiting step, directly or indirectly, in the formation of a particular nucleic acid.
W J Carpenter; J H Cherry
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  41     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1966 Jun 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-14    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  919-22     Citation Subset:  -    
Department of Horticulture, Purdue University, Lafayette, Indiana.
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