| Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells. | |
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MedLine Citation:
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PMID: 22721453 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Nanoparticles enter the cells through active processes, thanks to their capability of interacting with the cellular machinery. The protein layer (corona) which forms on their surface once nanoparticles are in contact with biologic fluids, such as the cell serum, mediates the interactions with cells in situ. As a consequence of this, here we show that the same nanomaterial can lead to very different biological outcomes, when exposed to cells in the presence or absence of a pre-formed corona. In particular, silica nanoparticles exposed to cells in absence of serum have a stronger adhesion to the cell membrane and higher internalisation efficiency, in comparison to what is observed in medium containing serum, when a pre-formed corona is present on their surface. The different exposure conditions not only affect the uptake levels, but also result in differences in the intracellular nanoparticle location and impact on cells. Interestingly, we also show that after only one hour of exposure, a corona of very different nature forms on the nanoparticles exposed to cells in absence of serum. Evidence suggests that these different outcomes can all be connected to the different adhesion and surface properties in the two conditions. |
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Authors:
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Anna Lesniak; Federico Fenaroli; Marco P Monopoli; Christoffer Aberg; Kenneth A Dawson; Anna Salvati |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-21 |
Journal Detail:
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Title: ACS nano Volume: - ISSN: 1936-086X ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101313589 Medline TA: ACS Nano Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
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