| Effects of preexercise feeding on markers of satellite cell activation. | |
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MedLine Citation:
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PMID: 20216467 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: The purpose of this study was to determine if consuming isoenergetic (25 g) doses of carbohydrate or protein versus a noncaloric placebo before conventional resistance training affected the myogenic expression of cell cycle-regulating genes as well as the muscle [DNA] acutely after exercise. METHODS: Ten untrained men (mean +/- SD: age = 22 +/- 4 yr, body mass = 77.8 +/- 8.3 kg, percent body fat = 17.8 +/- 4.0) participated in three resistance exercise sessions (three sets of 10 repetitions at 80% one-repetition maximum for the bilateral hack squat, leg press, and leg extension exercises) in a crossover fashion, which were preceded by carbohydrate, protein, or placebo ingestion 30 min before training. Presupplement/preexercise and 2- and 6-h postexercise muscle biopsies were obtained during each session and analyzed for fold changes in CDK4, CYCLIN D1, MGF, MYOD, P21(CIP1), and P27(KIP1) messenger RNA expression using real-time reverse transcriptase-polymerase chain reaction as well as muscle [DNA] using cuvette-based fluorometric methods. RESULTS: Nonparametric statistics were completed, and no conditions x time interaction effects were revealed. Several exercise-mediated responses were found to occur independent of condition: 1) muscle [DNA] increased at 6 h (+40%, P < 0.05), 2) CDK4 expression increased at 6 h (+86%, P < 0.05), 3) MYOD expression increased at 6 h (+98%, P < 0.05), 4) P27(KIP1) expression decreased at 2 h (j35%, P < 0.05) and 6 h (-59%, P < 0.001), and 5) P21(CIP1) expression substantially increased 2 and 6 h postexercise (+1.250% and +4.670%, respectively, P < 0.001). CONCLUSIONS: The tandem DNA and cell cycle regulator gene expression analyses provide preliminary evidence to suggest that satellite cell activation and proliferation may be occurring at early post-exercise time points after a conventional resistance exercise bout, a phenomenon that may seemingly be independent of preexercise macronutrient ingestion. |
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Authors:
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Michael D Roberts; Vincent J Dalbo; Scott E Hassell; Ryan Brown; Chad M Kerksick |
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Publication Detail:
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Type: Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Medicine and science in sports and exercise Volume: 42 ISSN: 1530-0315 ISO Abbreviation: Med Sci Sports Exerc Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-27 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8005433 Medline TA: Med Sci Sports Exerc Country: United States |
Other Details:
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Languages: eng Pagination: 1861-9 Citation Subset: IM; S |
Affiliation:
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Health and Exercise Science Department, University of Oklahoma, Norman, OK 73019-6081, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Biological Markers / metabolism Cyclin D1 / analysis Cyclin-Dependent Kinase 4 / analysis Cyclin-Dependent Kinase Inhibitor p21 / analysis Eating / physiology* Exercise / physiology* Gene Expression / physiology Humans Intracellular Signaling Peptides and Proteins / analysis Male Muscle Proteins / metabolism Muscle, Skeletal / metabolism MyoD Protein / analysis Resistance Training STAT5 Transcription Factor / analysis Satellite Cells, Skeletal Muscle / metabolism, physiology* Tumor Suppressor Proteins / analysis Young Adult |
| Chemical | |
Reg. No./Substance:
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0/Biological Markers; 0/CDKN1A protein, human; 0/CDKN1B protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Intracellular Signaling Peptides and Proteins; 0/Muscle Proteins; 0/MyoD Protein; 0/STAT5 Transcription Factor; 0/STAT5A protein, human; 0/Tumor Suppressor Proteins; 136601-57-5/Cyclin D1; EC 2.7.1.37/CDK4 protein, human; EC 2.7.11.22/Cyclin-Dependent Kinase 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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