Document Detail


Effects of a Porphyromonas gingivalis infection on inflammatory mediator response and pregnancy outcome in hamsters.
MedLine Citation:
PMID:  7927695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examines the effects of various localized, nondissemination challenges of Porphyromonas gingivalis on inflammatory mediator production and pregnancy outcome in the golden hamster. Live or heat-killed (HK) organisms were inoculated into a previously implanted subcutaneous tissue chamber on the 8th day of gestation to determine the effects on fetal weight, viability, and resorption. In one group of animals, HK organisms were inoculated prior to mating to determine the effects of previous exposure on day-8 gestational challenges. Chamber contents were assayed at 1 and 5 days after challenge for prostaglandin E2 (PGE2) and tumor necrosis factor alpha (TNF-alpha). All P. gingivalis challenges caused a significant increase in chamber PGE2 and TNF-alpha at P < 0.01 in the following order of potency: HK < Live < HK+Live. For example, following the HK+Live challenge, PGE2 levels increased from 4.7 pg/ml at baseline to 362 pg/ml at day 5 and TNF-alpha increased from 26.4 pg/ml to 724 pg/ml at day 5. The same order of potency of the various challenges was maintained with regard to the toxic effects of P. gingivalis on pregnancy outcome. For the HK+Live challenge, fetal weight was decreased 24%; embryolethality increased to 26.5% and the percent fetal resorption increased to 10.6% compared with control animal levels. There was a statistically significant association between increasing levels of both PGE2 and TNF-alpha and fetal growth retardation and embryolethality at P < 0.001. These data suggest that infections with gram-negative periodontal pathogens can elicit adverse pregnancy outcomes and that the levels of PGE2 and TNF-alpha produced as a result of challenge are associated with the severity of fetal effect.
Authors:
J G Collins; H W Windley; R R Arnold; S Offenbacher
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  62     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-04     Completed Date:  1994-11-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4356-61     Citation Subset:  IM    
Affiliation:
Dental Research Center, School of Dentistry, University of North Carolina at Chapel Hill 27599.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacteroidaceae Infections / complications,  metabolism*
Cricetinae
Dinoprostone / biosynthesis*
Female
Fetal Death / etiology
Fetal Resorption / etiology
Mesocricetus
Porphyromonas gingivalis*
Pregnancy
Pregnancy Complications, Infectious / metabolism*
Tumor Necrosis Factor-alpha / biosynthesis*
Grant Support
ID/Acronym/Agency:
R01-HD26652/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Tumor Necrosis Factor-alpha; 363-24-6/Dinoprostone
Comments/Corrections

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