| Effects of Plasmodium falciparum-infected erythrocytes on matrix metalloproteinase-9 regulation in human microvascular endothelial cells. | |
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MedLine Citation:
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PMID: 23375032 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the regulation of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human microvascular endothelium (HMEC-1) exposed to erythrocytes infected by different strains of Plasmodium falciparum (P. falciparum). METHODS: HMEC-1 cells were co-incubated for 72 h with erythrocytes infected by late stage trophozoite of D10 (chloroquine-sensitive) or W2 (chloroquine-resistant) P. falciparum strains. Cell supernatants were then collected and the levels of pro- or active gelatinases MMP-9 and MMP-2 were evaluated by gelatin zymography and densitometry. The release of pro-MMP-9, MMP-3, MMP-1 and TIMP-1 proteins was analyzed by western blotting and densitometry. RESULTS: Infected erythrocytes induced de novo proMMP-9 and MMP-9 release. Neither basal levels of proMMP-2 were altered, nor active MMP-2 was found. MMP-3 and MMP-1 secretion was significantly enhanced, whereas basal TIMP-1 was unaffected. All effects were similar for both strains. CONCLUSIONS: P. falciparum parasites, either chloroquine-sensitive or -resistant, induce the release of active MMP-9 protein from human microvascular endothelium, by impairing balances between proMMP-9 and its inhibitor, and by enhancing the levels of its activators. This work provides new evidence on MMP involvement in malaria, pointing at MMP-9 as a possible target in adjuvant therapy. |
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Authors:
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Sarah D'Alessandro; Nicoletta Basilico; Mauro Prato |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Asian Pacific journal of tropical medicine Volume: 6 ISSN: 1995-7645 ISO Abbreviation: Asian Pac J Trop Med Publication Date: 2013 Mar |
Date Detail:
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Created Date: 2013-02-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101533720 Medline TA: Asian Pac J Trop Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 195-9 Citation Subset: IM |
Copyright Information:
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Copyright © 2013 Hainan Medical College. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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Dipartimento di Sanità Pubblica-Microbiologia-Virologia, Università di Milano, Milano, Italy. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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