| Effects of Perhexiline-Induced Fuel Switch on the Cardiac Proteome and Metabolome. | |
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MedLine Citation:
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PMID: 23277191 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Perhexiline is a potent anti-anginal drug used for treatment of refractory angina and other forms of heart disease. It provides an oxygen sparing effect in the myocardium by creating a switch from fatty acid to glucose metabolism through partial inhibition of carnitine palmitoyltransferase 1 and 2. However, the precise molecular mechanisms underlying the cardioprotective effects elicited by perhexiline are not fully understood. The present study employed a combined proteomics, metabolomics and computational approach to characterise changes in murine hearts upon treatment with perhexiline. According to results based on difference in-gel electrophoresis, the most profound change in the cardiac proteome related to the activation of the pyruvate dehydrogenase complex. Metabolomic analysis by high-resolution nuclear magnetic resonance spectroscopy showed lower levels of total creatine and taurine in hearts of perhexiline-treated mice. Creatine and taurine levels were also significantly correlated in a cross-correlation analysis of all metabolites. Computational modelling suggested that far from inducing a simple shift from fatty acid to glucose oxidation, perhexiline may cause complex rebalancing of carbon and nucleotide phosphate fluxes, fuelled by increased lactate and amino acid uptake, to increase metabolic flexibility and to maintain cardiac output. |
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Authors:
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Xiaoke Yin; Joseph Dwyer; Sarah Langley; Ursula Mayr; Qiuru Xing; Ignat Drozdov; Adam Nabeebaccus; Ajay Shah; Basetti Madhu; John Griffiths; Lindsay M Edwards; Manuel Mayr |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-12-28 |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: - ISSN: 1095-8584 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 2012 Dec |
Date Detail:
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Created Date: 2013-1-1 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Ltd. |
Affiliation:
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King's BHF Centre, King's College London, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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