Document Detail


Effects of PUVA on a human skin epithelial cell line.
MedLine Citation:
PMID:  6116349     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An established human epithelial cell line was exposed to photoactivated 8-methoxy psoralen (PUVA) during exponential growth. Effects of PUVA treatment on cell growth were measured by cell kinetic methods (counting of cell numbers, flow cytometric measurements (FCM) of DNA and calculations of labelling indices (LI)). Doses of 8-methoxy psoralen and UVA were comparable to those used in patients. The cell number in PUVA treated cultures remained almost constant, and very few mitoses were seen for 144 h. About 9 h after PUVA, both FCM and LI showed an increase in the fraction of cells in S-phase, reaching a maximum of 85-90% after 24 h. DNA synthesis took place at a low rate in these cells. FCM showed an increasing fraction of polyploid cells after PUVA treatment. The possibility that inhibition of cell proliferation is one of the main effects of PUVA, is discussed.
Authors:
A S Johnsen; V Digernes; H E Rugstad
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Virchows Archiv. B, Cell pathology including molecular pathology     Volume:  37     ISSN:  0340-6075     ISO Abbreviation:  Virchows Arch., B, Cell Pathol.     Publication Date:  1981  
Date Detail:
Created Date:  1981-11-18     Completed Date:  1981-11-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9316922     Medline TA:  Virchows Arch B Cell Pathol Incl Mol Pathol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  61-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Count
Cell Division / drug effects
Cells, Cultured / drug effects
DNA / biosynthesis
Epithelium / drug effects
Humans
PUVA Therapy*
Photochemotherapy*
Skin / drug effects*
Time Factors
Chemical
Reg. No./Substance:
9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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