Document Detail


Effects of outer membrane protein TolC on the transport of Escherichia coli within saturated quartz sands.
MedLine Citation:
PMID:  23627691     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The outer membrane protein (OMP) TolC is the cell surface component of several drug efflux pumps that are responsible for bacterial resistance against a variety of antibiotics. In this research, we investigated the effects of OMP TolC on E. coli transport within saturated sands through column experiments using a wild-type E. coli K12 strain (with OMP TolC), as well as the corresponding transposon mutant (tolC::kan) and the markerless deletion mutant (ΔtolC). Our results showed OMP TolC could significantly enhance the transport of E. coli when the ionic strength was 20 mM NaCl or higher. The deposition rate coefficients for the wild-type E. coli strain (with OMP TolC) was usually >50% lower than those of the tolC-negative mutants. The measurements of contact angles using three probe liquids suggested that TolC altered the surface tension components of E. coli cells and lead to lower Hamaker constants for the cell-water-sand system. The interaction energy calculations using the extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) theory suggested that the deposition of the E. coli cell primarily occurred at the secondary energy minimum. The depth of the secondary energy minimum increased with ionic strength, and was greater for the TolC-deletion strains under high ionic strength conditions. Overall, the transport behavior of three E. coli strains within saturated sands could be explained by the XDLVO calculations. Results from this research suggested that antibiotic resistant bacteria expressing OMP TolC could spread more widely within sandy aquifers.
Authors:
Lucia Feriancikova; Sonia L Bardy; Lixia Wang; Jin Li; Shangping Xu
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-05-16
Journal Detail:
Title:  Environmental science & technology     Volume:  47     ISSN:  1520-5851     ISO Abbreviation:  Environ. Sci. Technol.     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-06-04     Completed Date:  2014-05-15     Revised Date:  2014-06-05    
Medline Journal Info:
Nlm Unique ID:  0213155     Medline TA:  Environ Sci Technol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5720-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Outer Membrane Proteins / genetics,  metabolism*
Biological Transport
Escherichia coli K12 / metabolism*
Escherichia coli Proteins / genetics,  metabolism*
Groundwater / microbiology
Membrane Transport Proteins / genetics,  metabolism*
Osmolar Concentration
Quartz*
Silicon Dioxide
Grant Support
ID/Acronym/Agency:
5R00GM083147-04/GM/NIGMS NIH HHS; R00 GM083147/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Outer Membrane Proteins; 0/Escherichia coli Proteins; 0/Membrane Transport Proteins; 0/tolC protein, E coli; 14808-60-7/Quartz; 7631-86-9/Silicon Dioxide
Comments/Corrections

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