Document Detail

Effects of oligo(3-hydroxyalkanoates) on the viability and insulin secretion of murine beta cells.
MedLine Citation:
PMID:  19723438     Owner:  NLM     Status:  MEDLINE    
Biopolyesters of polyhydroxyalkanoates (PHAs), including poly-3-hydroxybutyrate (PHB), co-polyester of 3-hydroxybutyrate and 4-hydroxybutyrate (P3HB4HB), and co-polyester of 3-hydroxybutyrate and 3-hydroxyhexanoate (PHBHHx) have been well investigated for their biocompatibility. For in vivo application, it is very important that the degradation products of PHAs, especially the oligomers, are not harmful to the cells and surrounding tissues. In this study, in vitro effects of oligo(3-hydroxybutyrate) (OHB), oligo(3-hydroxybutyrate-co-4-hydroxybutyrate) (O3HB4HB) and oligo(3-hydroxybutyrate-co-3-hydroxyhexanoate) (OHBHHx) on growth and differentiation of the murine beta cell line NIT-1 were investigated. Among the three oligo-hydroxyalkanoates (Oligo-HAs), cells treated with OHBHHx displayed higher viability, as measured by CCK-8 assay. Flow cytometric analysis of NIT-1 cells indicated that Oligo-HAs had an inhibitory effect on cell apoptosis. The cytosolic Ca(2+) transient of NIT-1 cells increased when fed with 0.04 g/l Oligo-HAs. For gap junction intercellular communication of cells, the effect of OHBHHx was the best among all materials tested. More importantly, extracellular insulin secretion was up-regulated after growing in OHBHHx for 48 h. The results demonstrated that the degradation products of PHAs, especially OHBHHx from PHBHHx, were not harmful to the beta cells. Therefore, PHBHHx warrant further study for application as a pancreatic tissue engineering material.
Xiao-Di Yang; Xiang-Hui Zou; Zhong-Wei Dai; Rong-Cong Luo; Chi-Ju Wei; Guo-Qiang Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomaterials science. Polymer edition     Volume:  20     ISSN:  1568-5624     ISO Abbreviation:  J Biomater Sci Polym Ed     Publication Date:  2009  
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-11-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9007393     Medline TA:  J Biomater Sci Polym Ed     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1729-46     Citation Subset:  IM    
Shantou University, Shantou 515063, Guangdong, People's Republic of China.
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MeSH Terms
Apoptosis / drug effects
Butyrates / chemistry*
Calcium / metabolism
Cell Communication / drug effects
Cell Line
Cell Survival / drug effects
Gap Junctions / drug effects,  metabolism
Insulin / secretion*
Insulin-Secreting Cells / cytology*,  drug effects,  metabolism,  secretion*
Materials Testing
Polymers / chemistry*,  pharmacology*
Tissue Engineering
Tissue Scaffolds / chemistry*
Reg. No./Substance:
0/Butyrates; 0/Polymers; 11061-68-0/Insulin; 7440-70-2/Calcium

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